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MW: 42,000
Extinction Coefficient: 1.0
Lyophilized from 2 mM Na phosphate, pH 6.5.
Storage:
Buy Purified Native Human Cathepsin D (CatD), Human Liver.
Bulk Qty Available.
Cathepsin D is an estrogen-regulated protein associated with tissue breakdown. Levels of cathepsin D have been positively correlated with recurring breast cancers of both node-negative and node-positive types. Additionally cathepsin D has been associated with amyloid formation in Alzheimer's plaques. As cathepsin D activity is increased by cigarette smoke, the enzyme may contribute to lung tissue damage in smokers.
Activity: >=300 units per mg protein. One unit is defined as the amount of enzyme that digests hemoglobin-releasing peptides which are soluble in 10% TCA. The reaction is measured by an increase of an A 280 of 1.0 per 60 minutes at 37°C . Substrate: acid denatured hemoglobin, pH 1.8 (0.2% in reaction mixture). Buffer: 100 mM formate, pH 3.3.
Purity: >=95% by SDS-PAGE
Prepared from tissue shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.
Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.
Product Citations:
Armstrong, Andrea, Niklas Mattsson, Hanna Appelqvist, Camilla Janefjord, Linnea Sandin, Lotta Agholme, Bob Olsson et al. "Lysosomal Network Proteins as Potential Novel CSF Biomarkers for Alzheimer’s Disease."?Neuromolecular medicine?(2013): 1-11
Appelqvist, Hanna, et al. "Lysosome-mediated apoptosis is associated with cathepsin D-specific processing of bid at Phe24, Trp48, and Phe183."Annals of Clinical & Laboratory Science?42.3 (2012): 231-242.
References:
Barrett, A.J. 1977. Proteinases in Mammalian Cells and Tissues. (A. Barrett, ed.) 4, 209.
Barrett, A.J. 1970. Biochemistry. 117, 601.
Tandon, A.K. et al. 1990. N. Engl. J. Med. 322, 297.
Cataldo, A.M. et al. 1990. Brain Res. 513, 181.
Chang, J.C. et al. Am. Rev. Respir. Dis. 140, 958.
