产品描述信息
MRA-231??Plasmodium falciparum, SP1 Primer(Primers and Probes)|Plasmodium falciparum|SP1 Primer|-20°C|MR4Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmodium falciparum, SP1 Primer, MRA-231, contributed by Malaria Research and Reference Reagent Resource Center (MR4)."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.This reagent was authenticated by the contributor. BEI Resources has not confirmed or validated this material.Primer SP1 is a twenty-one nucleotide primer designed for detection of the dihydrofolate reductase gene (dhfr) of Plasmodium falciparum when paired with a partner primer (MRA-228, MRA-229, MRA-230, MRA-235, MRA-236, MRA-237, MRA-238). The sequence of the SP1 21-mer is 5'-ATGATGGAACAAGTCTGCGAC-3'.Each vial contains between 10 ?g and 60 ?g of freeze-dried primer. Dissolving the contents of one vial (2.5 nmole) in 100 ?L of distilled water and mixing with its partner primer (total volume of 200 ?L), MRA-231 can be used for 200 PCR reactions (each reaction uses 1 ?L in a 25 ?L volume) at 500 nM. If used at 250 nM, 400 PCR reactions can be completed. If used at 250 nM, 400 reactions can be run. If three or four primers are used, the above volumes would be 1.5 and 2.0 ?L, respectively, per reaction.