MRA-199质粒FR-3D7,用于在恶性疟原虫中转染,在大肠杆菌中重组(质粒/载体）
产品图片
货号/SKU
MRA-199
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
MRA-199?? Plasmid FR-3D7, for Transfection in Plasmodium falciparum, Recombinant in Escherichia coli(Plasmid/Vectors)|Plasmodium falciparum|Plasmid FR-3D7, for Transfection in Plasmodium falciparum, Recombinant in Escherichia coli|4°C|CV PloweAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid FR-3D7, for Transfection in Plasmodium falciparum, Recombinant in Escherichia coli, MRA-199, contributed by Christopher V. Plowe."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
This reagent was authenticated by the contributor. BEI Resources has not confirmed or validated this material.
Dihydrofolate reductase (DHFR) from Plasmodium falciparum (P. falciparum), strain SL/D6 (pyrimethamine-sensitive) was amplified by primers FRY-71 (CGGGATCC TATGATGGAACAAGTCTGCG) and FRY-86 (GCGCGGCCGTCATATGACATGTATCTT). Note: The SL/D6 (pyrimethamine-sensitive) strain of P. falciparum has the same DHFRTS genotype as the 3D7 strain. Plasmid FR-3D7 has been submitted to serve as a control in allele-specific PCR for the analysis of mutations in P. falciparum DHFR. The relevant 3D7 DHFR genotype is as follows: 16A 50C 51N 59C 108S 164I. The resulting size of the plasmid is 6.508 kilobases.
Each vial of MRA-199 contains plasmid FR-3D7 in recombinant freeze-dried E. coli.
Plowe, C. V., et al. "Mutations in Plasmodium falciparum Dihydrofolate Reductase and Dihydropteroate Synthase and Epidemiologic Patterns of Pyrimethamine-Sulfadoxine Use and Resistance." J. Infect. Dis. 176 (1997): 1590-1596. PubMed: 9395372.
Wooden, J. M., et al. "Analysis in Yeast of Antimalaria Drugs that Target the Dihydrofolate Reductase of Plasmodium falciparum." Mol. Biochem. Parasitol. 85 (1997): 25-40. PubMed: 9108546.
Cortese, J. F. and C. V. Plowe. "Antifolate Resistance Due to New and Known Plasmodium falciparum Dihydrofolate Reductase Mutations Expressed in Yeast." Mol. Biochem. Parasitol. 94 (1998): 205-214. PubMed: 9747971.
This reagent was authenticated by the contributor. BEI Resources has not confirmed or validated this material.
Dihydrofolate reductase (DHFR) from Plasmodium falciparum (P. falciparum), strain SL/D6 (pyrimethamine-sensitive) was amplified by primers FRY-71 (CGGGATCC TATGATGGAACAAGTCTGCG) and FRY-86 (GCGCGGCCGTCATATGACATGTATCTT). Note: The SL/D6 (pyrimethamine-sensitive) strain of P. falciparum has the same DHFRTS genotype as the 3D7 strain. Plasmid FR-3D7 has been submitted to serve as a control in allele-specific PCR for the analysis of mutations in P. falciparum DHFR. The relevant 3D7 DHFR genotype is as follows: 16A 50C 51N 59C 108S 164I. The resulting size of the plasmid is 6.508 kilobases.
Each vial of MRA-199 contains plasmid FR-3D7 in recombinant freeze-dried E. coli.
Plowe, C. V., et al. "Mutations in Plasmodium falciparum Dihydrofolate Reductase and Dihydropteroate Synthase and Epidemiologic Patterns of Pyrimethamine-Sulfadoxine Use and Resistance." J. Infect. Dis. 176 (1997): 1590-1596. PubMed: 9395372.
Wooden, J. M., et al. "Analysis in Yeast of Antimalaria Drugs that Target the Dihydrofolate Reductase of Plasmodium falciparum." Mol. Biochem. Parasitol. 85 (1997): 25-40. PubMed: 9108546.
Cortese, J. F. and C. V. Plowe. "Antifolate Resistance Due to New and Known Plasmodium falciparum Dihydrofolate Reductase Mutations Expressed in Yeast." Mol. Biochem. Parasitol. 94 (1998): 205-214. PubMed: 9747971.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在此限制上订单将在发货前发送至NIAID进行批准. 这种试剂由贡献者认证. Bei资源尚未确认或验证过这种材料. 来自疟原虫（P. falciparum），菌株SL / D6（吡米甲胺敏感）的二氢酚酸还原酶（DHFR）通过引物FRY-71（CGGGATCC TATGATGGAACAAGTCTGCG）和FRY-86（GCGCGGCCGTCATATGACATCTTT）扩增.注意： p的sl / d6（嘧蓟敏感）菌株. Falciparum 具有与3D7应变相同的DHFRTS基因型.质粒FR-3D7已被提交以作为等位基因特异性PCR的对照，用于分析 p中的突变.恶毒膏 DHFR.相关的3D7 DHFR基因型如下：16A 50C 51N 59C 108S 164i.最终的质粒尺寸为6.508千碱基. 每瓶MRA-199含有重组冷冻干燥的质粒FR-3d7. Coli . Plowe，C. V.等人. “突变在”疟原虫疟原虫“中的二羟氢醇还原酶和二氢甲酸二丙酯合成酶和嘧霉素使用和抗性的流行病学模式.” j.感染. DIS. 176（1997）：1590-1596. PubMed：9395372. 木制，J. M.等人. “抗疟药酵母的酵母分析，其靶向疟原虫的二氢醇还原酶.” 摩尔.生物学习.寄生酚. 85（1997）：25-40. PubMed：9108546. Cortese，J. F.和C. V. Plowe. “由于新的和已知的抗性，抗雾化抗性，疟原虫疟原虫在酵母中表达的二氢醇还原酶突变.” 摩尔.生物学习.寄生醇. 94（1998）：205-214. PubMed：9747971.
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