NR-49697_N8神经氨酸酶(NA)流感病毒蛋白,A/江西-东湖/346/2013(H10N8),杆状病毒重组蛋白(蛋白）
产品图片
货号/SKU
NR-49697
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-49697?? N8 Neuraminidase (NA) Protein from Influenza Virus, A/Jiangxi-Donghu/346/2013 (H10N8), Recombinant from Baculovirus(Proteins)|Influenza A virus| N8 Neuraminidase (NA) Protein from Influenza Virus, A/Jiangxi-Donghu/346/2013 (H10N8), Recombinant from Baculovirus|-20°C or colder|F KrammerAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: N8 Neuraminidase (NA) Protein from Influenza Virus, A/Jiangxi-Donghu/346/2013 (H10N8), Recombinant from Baculovirus, NR-49697."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the N8 neuraminidase (NA) protein from influenza A virus, A/Jiangxi-Donghu/346/2013 (H10N8) was produced in Sf9 insect cells using a baculovirus expression vector system. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal six histidine tag followed by a tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site. The protein was purified by nickel affinity chromatography and then treated with thrombin to remove the tetramerization domain and the histidine tag. The thrombin-treated protein was further purified prior to final formulation. The full-length NA precursor protein is 470 residues (GISAID EpiFlu: EPI497479).
Each vial contains 50 ?g to 100 ?g of purified recombinant NA protein in 50 mM Tris buffer (pH 7.4) with 500 mM NaCl and 50% glycerol.
NR-49697 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2’-(4-methylumbelliferyl)-α-d-N-acetylneuraminic acid (4-MUNANA).
A recombinant form of the N8 neuraminidase (NA) protein from influenza A virus, A/Jiangxi-Donghu/346/2013 (H10N8) was produced in Sf9 insect cells using a baculovirus expression vector system. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal six histidine tag followed by a tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site. The protein was purified by nickel affinity chromatography and then treated with thrombin to remove the tetramerization domain and the histidine tag. The thrombin-treated protein was further purified prior to final formulation. The full-length NA precursor protein is 470 residues (GISAID EpiFlu: EPI497479).
Each vial contains 50 ?g to 100 ?g of purified recombinant NA protein in 50 mM Tris buffer (pH 7.4) with 500 mM NaCl and 50% glycerol.
NR-49697 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2’-(4-methylumbelliferyl)-α-d-N-acetylneuraminic acid (4-MUNANA).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. 一种重组形式
来自流感的N8神经氨酸酶（NA）蛋白来自流感病毒，A /江西 - 东湖/ 346/203
（H10N8）使用Baculovirus在SF9昆虫细胞中产生
表达式矢量系统.预测的
将Na基因的非统计素编码区域融合到合成基因段中
编码N-末端六个组氨酸标签，然后是四聚化结构域
从血管扩张剂刺激的磷蛋白（VASP）和凝血酶切割位点.通过镍亲和力纯化蛋白质
色谱法然后用凝血酶处理以除去四聚化
结构域和组氨酸标签.这
在最终制剂之前进一步纯化凝血酶处理的蛋白质.全长Na前体蛋白为470
残留物（GISAID EPIFLU：EPI497479）.＆nbsp; 每个
小瓶含有50μg至100μg纯化的重组Na蛋白，50mM Tris缓冲液（pH7.4），500mM NaCl
和50％甘油. NR-49697是
基于其切割的能力证明功能活跃
荧光底物2' - （4-甲基umb蛋糕）-α-D-N-乙酰尿蛋白酸
（4-MUNANA）.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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