A/江西-东湖/346/2013 (H10N8)

NR-49697?? N8 Neuraminidase (NA) Protein from Influenza Virus, A/Jiangxi-Donghu/346/2013 (H10N8), Recombinant from Baculovirus(Proteins)|Influenza A virus| N8 Neuraminidase (NA) Protein from Influenza Virus, A/Jiangxi-Donghu/346/2013 (H10N8), Recombinant from Baculovirus|-20°C or colder|F KrammerAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: N8 Neuraminidase (NA) Protein from Influenza Virus, A/Jiangxi-Donghu/346/2013 (H10N8), Recombinant from Baculovirus, NR-49697."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A recombinant form of the N8 neuraminidase (NA) protein from influenza A virus, A/Jiangxi-Donghu/346/2013 (H10N8) was produced in Sf9 insect cells using a baculovirus expression vector system. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal six histidine tag followed by a tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site.  The protein was purified by nickel affinity chromatography and then treated with thrombin to remove the tetramerization domain and the histidine tag. The thrombin-treated protein was further purified prior to final formulation. The full-length NA precursor protein is 470 residues (GISAID EpiFlu: EPI497479).   Each vial contains 50 ?g to 100 ?g of purified recombinant NA protein in 50 mM Tris buffer (pH 7.4) with 500 mM NaCl and 50% glycerol. NR-49697 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2’-(4-methylumbelliferyl)-α-d-N-acetylneuraminic acid (4-MUNANA).

NR-49441?? N8 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Jiangxi-Donghu/346/2013 (H10N8), Recombinant from Baculovirus(Proteins)|Influenza A virus| N8 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Jiangxi-Donghu/346/2013 (H10N8), Recombinant from Baculovirus|-20°C or colder|F KrammerAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: N8 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Jiangxi-Donghu/346/2013 (H10N8), Recombinant from Baculovirus, NR-49441."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.A recombinant form of the N8 neuraminidase (NA) protein from influenza A virus, A/Jiangxi-Donghu/346/2013 (H10N8) containing an N-terminal histidine tag was produced in Sf9 insect cells using a baculovirus expression vector system and was purified by nickel affinity chromatography. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal six histidine tag followed by a tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site. The full-length NA precursor protein is 470 residues (GISAID EpiFlu: EPI497479). Each vial contains 50 ?g to 150 ?g of purified recombinant NA protein in PBS (pH 7.4).