产品描述信息
NR-44081?? H7 Hemagglutinin (HA) Protein from Influenza A Virus, A/Anhui/1/2013 (H7N9), Recombinant from Baculovirus(Proteins)|Influenza A virus|H7 Hemagglutinin (HA) Protein from Influenza A Virus, A/Anhui/1/2013 (H7N9), Recombinant from Baculovirus|-20°C|P Palese, F Krammer, R HaiAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: H7 Hemagglutinin (HA) Protein from Influenza A Virus, A/Anhui/1/2013 (H7N9), Recombinant from Baculovirus, NR-44081."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the H7 hemagglutinin (HA) protein from influenza A virus, A/Anhui/1/2013 (H7N9) was produced in Sf9 insect cells using a baculovirus expression vector system. The predicted transmembrane and endodomain coding regions of the HA gene were replaced with a synthetic gene segment encoding a thrombin cleavage site, trimerizing (foldon) domain and six histidine residues, as described for several influenza A virus subtypes. The HA0 form of the protein was purified by nickel affinity chromatography and then treated with thrombin to remove the foldon domain and the hexa-histidine tag. The thrombin-treated protein was further purified prior to final formulation. The full-length HA precursor protein is 560 residues (GISAID EpiFlu: EPI439507).
Each vial contains 100 ?g to 200 ?g of purified recombinant HA protein in phosphate-buffered saline (PBS), pH 7.4 with 50% glycerol.