杆状病毒重组蛋白(蛋白），NR-1_Vaccinia virus

NR-49697?? N8 Neuraminidase (NA) Protein from Influenza Virus, A/Jiangxi-Donghu/346/2013 (H10N8), Recombinant from Baculovirus(Proteins)|Influenza A virus| N8 Neuraminidase (NA) Protein from Influenza Virus, A/Jiangxi-Donghu/346/2013 (H10N8), Recombinant from Baculovirus|-20°C or colder|F KrammerAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: N8 Neuraminidase (NA) Protein from Influenza Virus, A/Jiangxi-Donghu/346/2013 (H10N8), Recombinant from Baculovirus, NR-49697."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A recombinant form of the N8 neuraminidase (NA) protein from influenza A virus, A/Jiangxi-Donghu/346/2013 (H10N8) was produced in Sf9 insect cells using a baculovirus expression vector system. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal six histidine tag followed by a tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site.  The protein was purified by nickel affinity chromatography and then treated with thrombin to remove the tetramerization domain and the histidine tag. The thrombin-treated protein was further purified prior to final formulation. The full-length NA precursor protein is 470 residues (GISAID EpiFlu: EPI497479).   Each vial contains 50 ?g to 100 ?g of purified recombinant NA protein in 50 mM Tris buffer (pH 7.4) with 500 mM NaCl and 50% glycerol. NR-49697 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2’-(4-methylumbelliferyl)-α-d-N-acetylneuraminic acid (4-MUNANA).

NR-44364?? N9 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Shanghai/1/2013 (H7N9), Recombinant from Baculovirus(Proteins)|Influenza A virus|N9 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Shanghai/1/2013 (H7N9), Recombinant from Baculovirus|2°C to 8°C|P Palese, F Krammer, R HaiAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: N9 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Shanghai/1/2013 (H7N9), Recombinant from Baculovirus, NR-44364."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.A recombinant form of the N9 neuraminidase (NA) protein from influenza A virus, A/Shanghai/1/2013 (H7N9) containing an N-terminal histidine tag was produced in Sf9 insect cells using a baculovirus expression vector system and purified by nickel affinity chromatography. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal six histidine tag followed by a tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site. The full-length NA precursor protein is 465 residues (GISAID EpiFlu: EPI439487).Each vial contains 25 ?g to 75 ?g of purified recombinant NA protein in 50 mM Tris (pH 8.0), 500 mM NaCl, and 0.2% sodium azide.NR-44364 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2'-(4-methylumbelliferyl)-a-D-N-acetylneuraminic acid (4-MUNANA).