NR-3024含有猴痘病毒A35R基因的质粒pETMPOX/A33RoΔTM,扎伊尔79(质粒/载体）
产品图片
货号/SKU
NR-3024
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-3024?? Plasmid pETMPOX/A33RoΔTM Containing the A35R Gene from Monkeypox Virus, Zaire 79(Plasmid/Vectors)|Monkeypox virus|Plasmid pETMPOX/A33RoΔTM Containing the A35R Gene from Monkeypox Virus, Zaire 79|-20°C or colder|G FranchiniAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pETMPOX/A33RoΔTM Containing the A35R Gene from Monkeypox Virus, Zaire 79, NR-3024."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
The monkeypox A35R gene (GenBank AY160188) was amplified from plasmid pMPOX/A33Ro by PCR and subcloned into the prokaryotic expression vector pET26b (Novagen?), which contains coding sequences for a 6-histidine tag at the C-terminus of the expressed protein. The transmembrane (TM) domain of the A35R gene was deleted during subcloning so that the resulting vector would express only the extracellular domain of the protein. The plasmid was produced in Escherichia coli (E. coli) DH5a? cells and extracted using a QIAGEN? EndoFree? Plasmid Maxi Kit. The pETMPOX/A33Ro?TM plasmid is reported to direct the expression of monkeypox A35R protein in inclusion bodies when transformed into an appropriate expression host cell such as E. coli BL21(DE3). The designation A33Ro is used to indicate that the A35R gene is the monkeypox ortholog of the vaccinia virus A33R gene.
Each vial contains approximately 20 to 60 ng of plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0).
The monkeypox A35R gene (GenBank AY160188) was amplified from plasmid pMPOX/A33Ro by PCR and subcloned into the prokaryotic expression vector pET26b (Novagen?), which contains coding sequences for a 6-histidine tag at the C-terminus of the expressed protein. The transmembrane (TM) domain of the A35R gene was deleted during subcloning so that the resulting vector would express only the extracellular domain of the protein. The plasmid was produced in Escherichia coli (E. coli) DH5a? cells and extracted using a QIAGEN? EndoFree? Plasmid Maxi Kit. The pETMPOX/A33Ro?TM plasmid is reported to direct the expression of monkeypox A35R protein in inclusion bodies when transformed into an appropriate expression host cell such as E. coli BL21(DE3). The designation A33Ro is used to indicate that the A35R gene is the monkeypox ortholog of the vaccinia virus A33R gene.
Each vial contains approximately 20 to 60 ng of plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. 通过PCR从质粒pMpox / A330扩增猴子脂A35R基因（Genbank Ay160188）并将其亚克隆到原核表达载体pET26b中（ Novagen ?），其在表达蛋白的C-末端的C-末端含有6-组氨酸标签的编码序列.在亚克隆期间删除A35R基因的跨膜（TM）结构域，使得所得载体仅表达蛋白质的细胞外结构域.在大肠杆菌（大肠杆菌）中产生质粒，并使用QIAGEN ? endofree ?质粒maxi试剂盒提取.据报道PetMpox / A33RO？TM质粒在转化成适当的表达宿主细胞（例如 BL21（DE3）.指定A33RO用于表示A35R基因是痘苗病毒A33R基因的猴子曲氏菌. 每小时在TE缓冲液中含有约20至60ng的质粒DNA（10mM TRIS-HCl，1 mm EDTA，pH 7.0）.
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