产品描述信息
NR-3022?? Plasmid pETMPOX/A27Lo Containing the A29L Gene from Monkeypox Virus, Zaire 79(Plasmid/Vectors)|Monkeypox virus|Plasmid pETMPOX/A27Lo Containing the A29L Gene from Monkeypox Virus, Zaire 79|-20°C or colder|G FranchiniAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pETMPOX/A27Lo Containing the A29L Gene from Monkeypox Virus, Zaire 79, NR-3022."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.The monkeypox A29L gene (GenBank AY160186) was amplified from plasmid pMPOX/A27Lo by PCR and subcloned into the prokaryotic expression vector pET26b (Novagen?), which contains coding sequences for a 6-histidine tag at the C-terminus of the expressed protein. The resulting bacterial expression plasmid, pETMPOX/A27Lo, was produced in Escherichia coli (E. coli) DH5α? cells and extracted using a QIAGEN? EndoFree? Plasmid Maxi Kit. The pETMPOX/A27Lo plasmid is reported to direct the expression of soluble monkeypox A29L protein when transformed into an appropriate expression host cell such as E. coli BL21(DE3). The designation A27Lo is used to indicate that the A29L gene is the monkeypox ortholog of the vaccinia virus A27L gene.Each vial contains approximately 20 to 60 ng of plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0).