NR-3025含有猴痘病毒B6R基因的质粒pETMPOX/B5RoΔTM,扎伊尔79(质粒/载体）
产品图片
货号/SKU
NR-3025
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-3025?? Plasmid pETMPOX/B5RoΔTM Containing the B6R Gene from Monkeypox Virus, Zaire 79(Plasmid/Vectors)|Monkeypox virus|Plasmid pETMPOX/B5RoΔTM Containing the B6R Gene from Monkeypox Virus, Zaire 79|-20°C or colder|G FranchiniAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pETMPOX/B5RoΔTM Containing the B6R Gene from Monkeypox Virus, Zaire 79, NR-3025."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
The monkeypox B6R gene (GenBank AY160189) was amplified from plasmid pMPOX/B5Ro by PCR and subcloned into the prokaryotic expression vector pET26b (Novagen?), which contains coding sequences for a 6-histidine tag at the C-terminus of the expressed protein. The transmembrane (TM) domain of the B6R gene was deleted during subcloning so that the resulting vector would express only the extracellular domain of the protein. The plasmid was produced in Escherichia coli (E. coli) DH5a? cells and extracted using a QIAGEN? EndoFree? Plasmid Maxi Kit. The pETMPOX/B5Ro?TM plasmid is reported to direct the expression of monkeypox B6R protein in inclusion bodies when transformed into an appropriate expression host cell such as E. coli BL21(DE3). The designation B5Ro is used to indicate that the B6R gene is the monkeypox ortholog of the vaccinia virus B5R gene.
Each vial contains approximately 20 to 60 ng of plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0).
The monkeypox B6R gene (GenBank AY160189) was amplified from plasmid pMPOX/B5Ro by PCR and subcloned into the prokaryotic expression vector pET26b (Novagen?), which contains coding sequences for a 6-histidine tag at the C-terminus of the expressed protein. The transmembrane (TM) domain of the B6R gene was deleted during subcloning so that the resulting vector would express only the extracellular domain of the protein. The plasmid was produced in Escherichia coli (E. coli) DH5a? cells and extracted using a QIAGEN? EndoFree? Plasmid Maxi Kit. The pETMPOX/B5Ro?TM plasmid is reported to direct the expression of monkeypox B6R protein in inclusion bodies when transformed into an appropriate expression host cell such as E. coli BL21(DE3). The designation B5Ro is used to indicate that the B6R gene is the monkeypox ortholog of the vaccinia virus B5R gene.
Each vial contains approximately 20 to 60 ng of plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. 通过PCR从质粒pMPOX / B5RO扩增猴子类B6R基因（Genbank Ay160189）并将其亚克隆到原核表达载体pET26b中（ Novagen ?），其在表达蛋白的C-末端的C-末端含有6-组氨酸标签的编码序列.在亚克隆期间缺失B6R基因的跨膜（TM）结构域，使得所得载体仅表达蛋白质的细胞外结构域.在大肠杆菌（大肠杆菌）中产生质粒，并使用QIAGEN ? endofree ?质粒maxi试剂盒提取.据报道PetMpox / B5RO？TM质粒在转化成适当的表达宿主细胞（例如 BL21（DE3）.指定B5RO用于表明B6R基因是痘苗病毒B5R基因的猴子X滴. 每个小瓶在Te缓冲液中含有约20至60ng的质粒DNA（10mM Tris-HCl，1 mm EDTA，pH 7.0）.
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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