产品描述信息
NR-4705?? Genomic DNA from Yersinia pestis, Strain KIM Derivative 19 (D19)(Nucleic Acids)|Yersinia pestis|Genomic DNA from Yersinia pestis, Strain KIM Derivative 19 (D19)|-20°C or colder|RR BrubakerAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Yersinia pestis, Strain KIM Derivative 19 (D19), NR-4705."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.Genomic DNA was isolated from a preparation of Yersinia pestis (Y. pestis), strain KIM derivative 19 (D19).Y. pestis, strain KIM(D19) is an avirulent derivative of the highly virulent KIM strain, which was originally isolated from a Kurdistan Iran man (KIM). Y. pestis, strain KIM(D19) contains all three virulence plasmids, but lacks the unstable pgm locus. The complete sequence of the chromosome (4,600,755 bp; GenBank: AE009952), and plasmids; pMT1 (100,984 bp; GenBank: AF074611), pCD1 (70,504 bp; GenBank: AF074612), and pPCP1 (9,610 bp; GenBank: AF053945) from Y. pestis, strain KIM have been determined.The presence of the pMT1, pCD1, and pPCP1 plasmids in NR-4705 has been confirmed by PCR amplification of a virulence marker on each plasmid. NR-4705 has been qualified for PCR applications by amplification of approximately 1500 bp of the 16S ribosomal RNA gene as well as virulence marker sequences of approximately 1200, 1900, and 400 bp.Each vial contains approximately 4 to 6 ?g of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 7.4). The concentration is shown on the Certificate of Analysis. The vial should be centrifuged prior to opening.