NR-4727_来自鼠疫耶尔森菌的基因组DNA,菌株K25衍生物80(D80)(核酸）

NR-4727?? Genomic DNA from Yersinia pestis, Strain K25 Derivative 80 (D80)(Nucleic Acids)|Yersinia pestis|Genomic DNA from Yersinia pestis, Strain K25 Derivative 80 (D80)|-20°C or colder|RR BrubakerAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Yersinia pestis, Strain K25 Derivative 80 (D80), NR-4727."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.Yersinia pestis (Y. pestis), strain K25(D80) is an avirulent derivative of the K25 strain, a purine-auxotroph mutant of strain KIM-10. Y. pestis, strain K25(D80) contains the pMT1 and pPCP1 plasmids, but lacks the pCD1 plasmid that is essential for virulence, as well as the unstable chromosomal pgm locus.The presence of the pMT1 and pPCP1 plasmids in NR-4727 has been confirmed by PCR amplification of a virulence marker on each plasmid. NR-4727 has been qualified for PCR applications by amplification of approximately 1500 bp of the 16S ribosomal RNA gene, 800 bp of a Y. pestis specific sequence, as well as virulence marker sequences of approximately 1200 and 400 bp.Each vial contains approximately 4 to 6 ?g of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 7.4).