大肠杆菌重组(抗原制剂），NR-1_Vaccinia virus

NR-4676?? Shiga Toxin Type 2 Toxoid, Recombinant from Escherichia coli(Antigen Preparations)|Escherichia coli|Shiga Toxin Type 2 Toxoid, Recombinant from Escherichia coli|-20°C or colder|AD O'Brien, JF SinclairAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Shiga Toxin Type 2 Toxoid, Recombinant from Escherichia coli, NR-4676."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.NR-4676 is a recombinant toxoid of Shiga toxin type 2 (Stx2) with genetic mutations in the catalytic A subunit which render the protein non-toxic. The recombinant B subunit includes a C-terminal hexa-histidine tag. The recombinant toxoid was expressed in E. coli and purified by nickel affinity chromatography. NR-4676 has a theoretical molecular weight of approximately 33080 daltons for subunit A and 8640 daltons for subunit B. The predicted amino acid sequence of NR-4676 is shown in Table 1 (Subunit A) and Table 2 (Subunit B) on the product sheet.The sequences of the structural genes for Shiga toxin from Shigella dysenteriae and Shiga toxin type 2 from Escherichia coli (E. coli) have been determined. The crystal structure of Shiga toxin from Shigella dysenteriae and Shiga toxin type 2 from E. coli have been solved (PDB: 1DM0 and 1R4P, respectively).Each vial of NR-4676 contains approximately 50 ?g of recombinant Stx2 toxoid suspended in phosphate buffered saline.NR-4676 reacts with rabbit polyclonal antibody to Stx2 and is not cytotoxic in Vero cells.