产品图片
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货号/SKU
NR-4709
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-4709?? Genomic DNA from Yersinia pestis, Strain KIM Derivative 23 (D23)(Nucleic Acids)|Yersinia pestis|Genomic DNA from Yersinia pestis, Strain KIM Derivative 23 (D23)|-20°C or colder|RR BrubakerAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Yersinia pestis, Strain KIM Derivative 23 (D23), NR-4709."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Yersinia pestis (Y. pestis), strain KIM(D23) is an avirulent derivative of the highly virulent KIM strain, which was originally isolated from a Kurdistan Iran man (KIM). Y. pestis, strain KIM(D23) contains the pMT1 plasmid, but lacks the pCD1 and pPCP1 plasmids that are essential for virulence, as well as unstable chromosomal pgm locus. The complete sequence of the chromosome (4,600,755 bp; GenBank: AE009952), pMT1 (100,984 bp; GenBank: AF074611), pCD1 (70,504 bp; GenBank: AF074612), and pPCP1 (9,610 bp; GenBank: AF053945) from Y. pestis KIM have been determined.
The presence of the pMT1 plasmid in NR-4709 has been confirmed by PCR amplification of a virulence marker on plasmid. NR-4709 has been qualified for PCR applications by amplification of approximately 1500 bp of the 16S ribosomal RNA gene, 800 bp of a Y. pestis specific sequence, as well as the virulence marker sequence of approximately 1200 bp.
Each vial contains approximately 4 to 6 ?g of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 7.4).
Yersinia pestis (Y. pestis), strain KIM(D23) is an avirulent derivative of the highly virulent KIM strain, which was originally isolated from a Kurdistan Iran man (KIM). Y. pestis, strain KIM(D23) contains the pMT1 plasmid, but lacks the pCD1 and pPCP1 plasmids that are essential for virulence, as well as unstable chromosomal pgm locus. The complete sequence of the chromosome (4,600,755 bp; GenBank: AE009952), pMT1 (100,984 bp; GenBank: AF074611), pCD1 (70,504 bp; GenBank: AF074612), and pPCP1 (9,610 bp; GenBank: AF053945) from Y. pestis KIM have been determined.
The presence of the pMT1 plasmid in NR-4709 has been confirmed by PCR amplification of a virulence marker on plasmid. NR-4709 has been qualified for PCR applications by amplification of approximately 1500 bp of the 16S ribosomal RNA gene, 800 bp of a Y. pestis specific sequence, as well as the virulence marker sequence of approximately 1200 bp.
Each vial contains approximately 4 to 6 ?g of bacterial genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH ~ 7.4).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. yersinia pestis(Y.pestis),菌株金(d23)是一种无毒的衍生物高毒性的金菌株,最初是从库尔德斯坦伊朗人(Kim)的孤立的. y. Pestis ,菌株Kim(D23)含有PMT1质粒,但缺乏对毒力至毒力至关重要的PCD1和PPCP1质粒,以及不稳定的染色体 PGM 基因座.染色体的完整序列(4,600,755 bp; genbank:ae009952),pmt1(100,984 bp; genbank:af074611),pcd1(70,504 bp; genbank:af074612),ppcp1(9,610bp; genbank:af053945)从 y .已经确定了Pestis Kim. 在质粒上的毒力标记物的PCR扩增证实了NR-4709中PMT1质粒的存在.通过扩增16S核糖体RNA基因的约1500bp,800bp的αy,NR-4709已获得PCR应用. Pestis 特定序列,以及约1200bp的毒力标记序列. TE缓冲液中的细菌基因组DNA约为4至6μg(10mm tris-HCl,1mm EDTA,pH?7.4).
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
品牌标识
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