产品描述信息
MRA-1301??Anopheles coluzzii, QUAS-CD8:GFP, Eggs(Vectors)|Anopheles coluzzii|QUAS-CD8:GFP, Eggs| 24°C to 30°C, 50 to 80% relative humidity|CJ PotterAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Anopheles coluzzii, Strain QUAS-CD8:GFP, Eggs, MRA-1301, contributed by Christopher J. Potter.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
The Anopheles coluzzii (An. coluzzii) colony originated in Ngousso, Cameroon.
An. coluzzii, strain QUAS-CD8:GFP is a new transgenic strain utilizing the binary expression system QF-QUAS (Q-system). The strain was produced using a piggyBac vector containing cyan fluorescent protein (CFP) under control of the synthetic eye promoter 3xP3. QUAS is an enhancer element for the transcriptional activator QF2, originally from Neurospora crassa (fungus). Strain QUAS-CD8:GFP is an effector construct that contains 15 copies of the QUAS sequence, enhancing expression of even weak promoters, as well as mouse CD8, a single-pass membrane protein, fused to green fluorescent protein (GFP) to allow membrane-targeted visualization. This transgenic line can be used in combination with promoter-QF2 driver constructs to express CD8:GFP in specific tissues.
Note: MRA-1301 is a Q-system effector line, and must be crossed with a Q-system driver line for tissue-specific gene expression. MRA-1301 can be crossed with MRA-1300.
MRA-1301 contains a suitable number of eggs to establish a stock. Eggs are provided on damp filter paper and should be hatched immediately upon receipt.