MRA-1301按蚊,QUAS-CD8：GFP,鸡蛋(矢量）
产品图片
货号/SKU
MRA-1301
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
MRA-1301??Anopheles coluzzii, QUAS-CD8:GFP, Eggs(Vectors)|Anopheles coluzzii|QUAS-CD8:GFP, Eggs| 24°C to 30°C, 50 to 80% relative humidity|CJ PotterAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Anopheles coluzzii, Strain QUAS-CD8:GFP, Eggs, MRA-1301, contributed by Christopher J. Potter.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
The Anopheles coluzzii (An. coluzzii) colony originated in Ngousso, Cameroon.
An. coluzzii, strain QUAS-CD8:GFP is a new transgenic strain utilizing the binary expression system QF-QUAS (Q-system). The strain was produced using a piggyBac vector containing cyan fluorescent protein (CFP) under control of the synthetic eye promoter 3xP3. QUAS is an enhancer element for the transcriptional activator QF2, originally from Neurospora crassa (fungus). Strain QUAS-CD8:GFP is an effector construct that contains 15 copies of the QUAS sequence, enhancing expression of even weak promoters, as well as mouse CD8, a single-pass membrane protein, fused to green fluorescent protein (GFP) to allow membrane-targeted visualization. This transgenic line can be used in combination with promoter-QF2 driver constructs to express CD8:GFP in specific tissues.
Note: MRA-1301 is a Q-system effector line, and must be crossed with a Q-system driver line for tissue-specific gene expression. MRA-1301 can be crossed with MRA-1300.
MRA-1301 contains a suitable number of eggs to establish a stock. Eggs are provided on damp filter paper and should be hatched immediately upon receipt.
The Anopheles coluzzii (An. coluzzii) colony originated in Ngousso, Cameroon.
An. coluzzii, strain QUAS-CD8:GFP is a new transgenic strain utilizing the binary expression system QF-QUAS (Q-system). The strain was produced using a piggyBac vector containing cyan fluorescent protein (CFP) under control of the synthetic eye promoter 3xP3. QUAS is an enhancer element for the transcriptional activator QF2, originally from Neurospora crassa (fungus). Strain QUAS-CD8:GFP is an effector construct that contains 15 copies of the QUAS sequence, enhancing expression of even weak promoters, as well as mouse CD8, a single-pass membrane protein, fused to green fluorescent protein (GFP) to allow membrane-targeted visualization. This transgenic line can be used in combination with promoter-QF2 driver constructs to express CD8:GFP in specific tissues.
Note: MRA-1301 is a Q-system effector line, and must be crossed with a Q-system driver line for tissue-specific gene expression. MRA-1301 can be crossed with MRA-1300.
MRA-1301 contains a suitable number of eggs to establish a stock. Eggs are provided on damp filter paper and should be hatched immediately upon receipt.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
anopheles coluzzii（a.孔子）群落起源于喀麦隆Ngousso.
一个. Coluzzii ，菌株quas-CD8：GFP是利用二元表达系统QF- QUAs （Q-System）的新的转基因应变.在合成眼启动子的控制下，使用含有青色荧光蛋白（CFP）的Piggybac载体产生菌株 3xp3 / i>. quas 是转录活化剂qf2的增强元素，最初来自神经孢子crassa（真菌）.菌株QUAS-CD8：GFP是含有15个拷贝的 quas 序列的效应构建体，增强甚至弱启动子的表达，以及小鼠CD8，单通膜蛋白，融合到绿色荧光灯蛋白质（GFP）允许膜靶向可视化.该转基因系可以与启动子-QF2驱动器构建体组合使用，以表达特定组织中的CD8：GFP.
注意：MRA-1301是一种Q系统效应线，必须与Q-System驱动线交叉以进行组织特异性基因表达. MRA-1301可以与MRA-1300交叉.
MRA-1301含有合适数量的鸡蛋来建立股票.鸡蛋提供潮湿的滤纸上，并在收到后立即孵化.
厂牌介绍
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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