产品描述信息
NR-19678?? Mycobacterium tuberculosis Gateway? Clone Set, Recombinant in Escherichia coli, Plate 42(Clones)|Mycobacterium tuberculosis|Mycobacterium tuberculosis Gateway? Clone Set, Recombinant in Escherichia coli, Plate 42|-80°C or colder|Pathogen Functional Genomics Resource Center at the J. Craig Venter InstituteAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis Gateway
? Clone Set, Recombinant in Escherichia coli, Plate 42, NR-19678."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.The Mycobacterium tuberculosis (M. tuberculosis) Gateway
? clone set consists of 42 plates which contain 3724 sequence validated clones (3294 M. tuberculosis, strain H37Rv clones supplemented with 430 unique open reading frames (ORF) from M. tuberculosis, strain CDC1551) cloned in Escherichia coli (E. coli) DH10B-T1 cells. Each ORF was recombined in vector
pDONR?221 with an ATG start codon and no stop codon. The sequence was validated by full length sequencing of each entry clone with greater than 1X coverage and a mutation rate of less than 0.2%.Information related to the use of Gateway
? Clones can be obtained from
Invitrogen?. Recombination was facilitated through an attB substrate (attB-PCR product or a linearized attB expression clone) with an attP substrate (pDONR?221) to create an attL-containing entry clone. The entry clone contains recombinational cloning sites, attL1 and attL2 to facilitate gene transfer into a destination vector, M13 forward and reverse priming sites for sequencing and a kanamycin resistance gene for selection. Please refer to the Invitrogen?
Gateway? Technology Manual for additional details.Plate orientation and viability were confirmed for NR-19678.Each well of the 96-well plate contains approximately 40 ?L of E. coli culture (strain DH10B-T1) in Luria Bertani (LB) Broth containing 50 ?g/mL kanamycin supplemented with 15% glycerol.Clone plates are replicated using a BioMek
? FX robot. Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources only confirms the clone plate orientation and viability of randomly picked clones. BEI Resources does not confirm or validate individual clone identities provided by the contributor.