NR-19458土拉弗朗西斯菌亚种.土拉菌,菌株SCHUS4,Gateway?克隆集,在大肠杆菌中重组,板1(克隆)
The Francisella tularensis (F. tularensis) subsp. tularensis, strain SCHU S4, Gateway? clone set consists of 19 plates which contain 1693 sequence validated clones from F. tularensis subsp. tularensis, strain SCHU S4 cloned in Escherichia coli (E. coli) DH10B-T1 cells. Each open reading frame was constructed in vector pDONR?221 (Invitrogen?) with a native start codon and no stop codon. The sequence was validated by full length sequencing of each clone with greater than 1X coverage and a mutation rate of less than 0.2%.
Information related to the use of Gateway? Clones can be obtained from Invitrogen?. Recombination was facilitated through an attB substrate (attB-PCR product or a linearized attB expression clone) with an attP substrate (pDONR?221) to create an attL-containing entry clone. The entry clone contains recombinational cloning sites, attL1 and attL2 to facilitate gene transfer into a destination vector, M13 forward and reverse priming sites for sequencing and a kanamycin resistance gene for selection. Please refer to the Invitrogen? Gateway? Technology Manual for additional details.
Each inoculated well of the 96-well plate contains approximately 60 ?L of E. coli culture (strain DH10B-T1) in Luria Bertani (LB) Broth containing 50 ?g/mL kanamycin supplemented with 15% glycerol.
Note: Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources cannot confirm or validate any clone not identified on the plate information table found on the Product Sheet.
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