NR-19742_炭疽杆菌Gateway?克隆套装,在大肠杆菌中重组,板18(克隆）
产品图片
货号/SKU
NR-19742
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-19742?? Bacillus anthracis Gateway? Clone Set, Recombinant in Escherichia coli, Plate 18(Clones)|Bacillus anthracis|Bacillus anthracis Gateway? Clone Set, Recombinant in Escherichia coli, Plate 18|-80°C or colder|Pathogen Functional Genomics Resource Center at the J. Craig Venter InstituteAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Bacillus anthracis Gateway? Clone Set, Recombinant in Escherichia coli, Plate 18, NR-19742."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
The Bacillus anthracis (B. anthracis) Gateway? clone set consists of 58 plates which contain 5341 sequence validated clones from B. anthracis, strains Ames (5139 clones), Sterne (107 clones; contains plasmid pXO1 only) and A2012 (95 clones; contains plasmid pXO2 only) cloned in Escherichia coli (E. coli) DH10B-T1 cells. Each open reading frame was constructed in vector pDONR?221 (Invitrogen?) with a native start codon and no stop codon. The library was independently cloned and sequence verified by the Harvard Institute of Proteomics.
Information related to the use of Gateway? Clones can be obtained from Invitrogen?. Recombination was facilitated through a Harvard-modified attB substrate (attB-PCR product or a linearized attB expression clone) with an attP substrate (pDONR?221) to create an attL-containing entry clone. The entry clone contains recombinational cloning sites, attL1 and attL2 to facilitate gene transfer into a destination vector, M13 forward and reverse priming sites for sequencing and a kanamycin resistance gene for selection. Please refer to the Invitrogen? Gateway? Technology Manual for additional details.
Plate orientation and viability were confirmed for NR-19742.
Each inoculated well of the 96-well plate contains approximately 60 ?L of E. coli culture (strain DH10B-T1) in Luria Bertani (LB) broth containing 50 ?g/mL kanamycin supplemented with 15% glycerol.
Clone plates are replicated using a BioMek? FX robot. Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources only confirms the clone plate orientation and viability of randomly picked clones. BEI Resources does not confirm or validate individual clone identities provided by the contributor.
The Bacillus anthracis (B. anthracis) Gateway? clone set consists of 58 plates which contain 5341 sequence validated clones from B. anthracis, strains Ames (5139 clones), Sterne (107 clones; contains plasmid pXO1 only) and A2012 (95 clones; contains plasmid pXO2 only) cloned in Escherichia coli (E. coli) DH10B-T1 cells. Each open reading frame was constructed in vector pDONR?221 (Invitrogen?) with a native start codon and no stop codon. The library was independently cloned and sequence verified by the Harvard Institute of Proteomics.
Information related to the use of Gateway? Clones can be obtained from Invitrogen?. Recombination was facilitated through a Harvard-modified attB substrate (attB-PCR product or a linearized attB expression clone) with an attP substrate (pDONR?221) to create an attL-containing entry clone. The entry clone contains recombinational cloning sites, attL1 and attL2 to facilitate gene transfer into a destination vector, M13 forward and reverse priming sites for sequencing and a kanamycin resistance gene for selection. Please refer to the Invitrogen? Gateway? Technology Manual for additional details.
Plate orientation and viability were confirmed for NR-19742.
Each inoculated well of the 96-well plate contains approximately 60 ?L of E. coli culture (strain DH10B-T1) in Luria Bertani (LB) broth containing 50 ?g/mL kanamycin supplemented with 15% glycerol.
Clone plates are replicated using a BioMek? FX robot. Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources only confirms the clone plate orientation and viability of randomly picked clones. BEI Resources does not confirm or validate individual clone identities provided by the contributor.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. Bacillus Anthracis（B. anthracis）网关?克隆集由58平板组成，含有来自 b的5341序列验证的克隆.炭疽病，菌株ames（5139克隆），斯托纳（107个克隆;仅含有质粒pXO1）和A2012（95克隆;仅含有质粒pXO2）在大肠杆菌（大肠杆菌）中克隆DH10B-T1细胞.每个开放阅读框架是在矢量 pdonr?221 （ Invitrogen ?），具有本机起始密码子和没有停止密码子.图书馆被哈佛蛋白质组学研究所所验证的ronsed和序列. 相关信息网关?克隆可以从 Invitrogen ?.通过哈维德改性的 ATT B底物（ att B-PCR产物或线性化的 att B表达克隆），促进重组.用 ATT P底板（PDONR?221），以产生含有含有ATT L的入口克隆.进入克隆含有重组克隆位点， att l1和 att L2，以促进基因转移到目的载体中，用于测序和卡那霉素抗性基因的目的载体，M13向前和逆向引发位点选择.请参阅Invitrogen?网关?技术手册更多详情. NR-19742确认了板取向和可行性. 96孔板的每个接种井含有约60μl的 e. Coli 培养（菌株DH10B-T1）在含有50μg/ mL卡那霉素的LURIA Bertani（LB）肉汤中，其补充有15％甘油. 克隆板被复制使用生物滤泡? fx机器人. 96孔格式的生产增加了相邻井之间的交叉污染风险.应纯化各个克隆（例如，使用良好的微生物实践的单菌落分离和纯化）并在使用前进行序列验证. Bei Resources仅确认随机挑选的克隆的克隆板取向和可存度. Bei资源不确认或验证贡献者提供的单个克隆身份.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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