NR-19793产肠毒素大肠杆菌表达克隆集,大肠杆菌重组,平板4(克隆）
产品图片
货号/SKU
NR-19793
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、2
产品描述信息
NR-19793?? Enterotoxigenic Escherichia coli Expression Clone Set, Recombinant in Escherichia coli, Plate 4(Clones)|Escherichia coli|Enterotoxigenic Escherichia coli Expression Clone Set, Recombinant in Escherichia coli, Plate 4|-80°C or colder|Pathogen Functional Genomics Resource Center at the J. Craig Venter InstituteAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Enterotoxigenic Escherichia coli Expression Clone Set, Recombinant in Escherichia coli, Plate 4, NR-19793."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
The Enterotoxigenic Escherichia coli (ETEC) expression clone set consists of approximately 900 sequence validated clones from Escherichia coli (E. coli) strains H10407, E24377A and B7A cloned in E. coli DH10B-T1 cells. Each open reading frame was constructed, using ligation independent cloning, in vector pMCSG7 (a pET21 derivative that contains an N-terminal 6xHis tag; for routine HTP purification). The sequence was validated by full length sequencing of each clone (using 5' and 3' primers; TACTTCCAATCC AATGCG and TTATCC ACTTCCAATG, respectively) with greater than 1X coverage and a mutation rate of less than 0.2%.
Each inoculated well of the 96-well plate contains approximately 60 ?L of E. coli culture (strain DH10B-T1) in Luria Bertani (LB) Broth containing 100 ?g/mL ampicillin supplemented with 15% glycerol.
Note: Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources cannot confirm or validate any clone not identified on the plate information table found on the Product Information Sheet.
The Enterotoxigenic Escherichia coli (ETEC) expression clone set consists of approximately 900 sequence validated clones from Escherichia coli (E. coli) strains H10407, E24377A and B7A cloned in E. coli DH10B-T1 cells. Each open reading frame was constructed, using ligation independent cloning, in vector pMCSG7 (a pET21 derivative that contains an N-terminal 6xHis tag; for routine HTP purification). The sequence was validated by full length sequencing of each clone (using 5' and 3' primers; TACTTCCAATCC AATGCG and TTATCC ACTTCCAATG, respectively) with greater than 1X coverage and a mutation rate of less than 0.2%.
Each inoculated well of the 96-well plate contains approximately 60 ?L of E. coli culture (strain DH10B-T1) in Luria Bertani (LB) Broth containing 100 ?g/mL ampicillin supplemented with 15% glycerol.
Note: Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources cannot confirm or validate any clone not identified on the plate information table found on the Product Information Sheet.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID进行批准. （ETEC）表达式克隆集由约900个序列验证的克隆组成I>大肠杆菌（大肠杆菌）菌株H10407，E24377A和B7A克隆在 E中. COLI DH10B-T1细胞.使用连接独立克隆，在向量 pmcsg7 （a pet21衍生物包含N末端6xHIS标签;用于常规HTP净化）.通过每个克隆（使用5'和3'引物的全长测序验证序列; TactcTcaatcc AATGCG和TTATCC Acttccaatg，覆盖率大于1倍，突变率小于0.2％. 96孔板的接种阱含有约60μl的. Coli 培养（菌株DH10B-T1）在含有100μg/ mL氨苄青霉素的LURIA Bertani（LB）肉汤中，补充有15％甘油. 注意：生产96孔格式增加了相邻井之间的交叉污染的风险.应纯化各个克隆（例如，使用良好的微生物实践的单菌落分离和纯化）并在使用前进行序列验证. Bei Resources无法确认或验证产品信息表上的板信息表上未识别的任何克隆.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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