产品描述信息
NR-19696?? Vibrio cholerae Gateway? Clone Set, Recombinant in Escherichia coli, Plate 18(Clones)|Vibrio cholerae|Vibrio cholerae Gateway? Clone Set, Recombinant in Escherichia coli, Plate 18|-80°C or colder|Pathogen Functional Genomics Resource Center at the J. Craig Venter InstituteAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vibrio cholerae Gateway
? Clone Set, Recombinant in Escherichia coli, Plate 18, NR-19696."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.The Vibrio cholerae (V. cholerae) Gateway
? clone set consists of 46 plates which contain 3813 sequence validated clones from V. cholerae, strain El Tor N16961 cloned in Escherichia coli (E. coli) DH10B-T1 cells. Each open reading frame was constructed in vector
pDONR?221 with a native start codon and stop codon. The library was independently cloned and sequence verified by the Harvard Institute of Proteomics.Information related to the use of Gateway
? Clones can be obtained from
Invitrogen?. Recombination was facilitated through an attB substrate (attB-PCR product or a linearized attB expression clone) with an attP substrate (pDONR?221) to create an attL-containing entry clone. The entry clone contains recombinational cloning sites, attL1 and attL2 to facilitate gene transfer into a destination vector, M13 forward and reverse priming sites for sequencing and a kanamycin resistance gene for selection. Please refer to the Invitrogen?
Gateway? Technology Manual for additional details.Each inoculated well of the 96-well plate contains approximately 60 ?L of E. coli culture (strain DH10B-T1) in Luria Bertani (LB) broth containing 50 ?g/mL kanamycin supplemented with 15% glycerol.
Note: Production
in the 96-well format has increased risk of cross-contamination between
adjacent wells. Individual clones should be purified (e.g. single colony
isolation and purification using good microbiological practices) and
sequence-verified prior to use. BEI Resources does not confirm or validate
individual mutants provided by the contributor.
