NR-52520含有ZsGreen基因的载体pHAGE2(质粒/载体）

NR-52520?? Vector pHAGE2 Containing the ZsGreen Gene(Plasmid/Vectors)||Vector pHAGE2 Containing the ZsGreen Gene| -20°C or colder|J Bloom, A BalaszAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Vector pHAGE2 Containing the ZsGreen Gene, NR-52520.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. The vector includes the green fluorescent protein (GFP) gene ZsGreen1, which was subcloned into the pHAGE2 lentiviral backbone vector under the CMV promoter. In addition, pHAGE2 includes the Woodchuck hepatitis virus post-transcriptional regulatory element to enhance levels of transcription and gene expression. NR-52520 contains the beta-lactamase gene, bla, to provide transformant selection through ampicillin resistance in Escherichia coli (E. coli). The resulting size of the plasmid is approximately 7070 base pairs. The complete plasmid sequence and map are provided on the BEI Resources webpage. The plasmid was produced in E. coli and extracted. NR-52520 is part of a lentiviral expression system, and additional BEI Resources items are required for successful expression. Lentiviral expression requires lentiviral helper plasmids (BEI Resources NR-52517, NR-52518 and NR-52519; kits NR-53816 and NR-53817) and a viral entry protein (BEI Resources NR-52513, NR-52514, NR-52515, NR-53742 or NR-53765). Protocols for the use of these items are published. Note: NR-52520 does not include an antibiotic selection cassette for mammalian expression. The ZsGreen1 gene is derived from the reef coral Zoanthus sp. GFP and has been codon optimized for mammalian expression and engineered for brighter fluorescence. Each vial contains plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0). The vial should be centrifuged prior to opening. Note: The contents of the vial should be used to replicate the plasmid in E. coli prior to mammalian expression. ZsGreen For information on purchasing a license to use this product for research (for-profit entities) or commercial purposes (any entity) other than those permitted in the limited use label license, please contact a licensing representative at Takara Bio USA, Inc., by phone at 650.919.7320 or by e-mail at licensing@takarabio.com.