菌株VK1(蛋白质），NR-1_Vaccinia virus

MRA-52?? Plasmodium gallinaceum 28 kDa Sexual Stage Surface Protein yPgs28.1, Recombinant from Saccharomyces cerevisiae, Strain VK1(Proteins)|Plasmodium gallinaceum|Plasmodium gallinaceum 28 kDa Sexual Stage Surface Protein yPgs28.1, Recombinant from Saccharomyces cerevisiae, Strain VK1|-70°C|DC KaslowAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmodium gallinaceum 28 kDa Sexual Stage Surface Protein yPgs28.1, Recombinant from Saccharomyces cerevisiae, Strain VK1, MRA-52, contributed by David C. Kaslow."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.This reagent was authenticated by the contributor. BEI Resources has not confirmed or validated this material.Yeast-secreted recombinant 28 kDa sexual-stage surface protein from Plasmodium gallinaceum (P. gallinaceum).The protein is expressed from the open reading frame of Pgs28 from P. gallinaceum, isolate 8A as a recombinant protein in Saccharomyces cerevisiae, strain VK1 using the pIXY154 expression plasmid. The protein expression begins at Ala22 and has a carboxyl terminal His6 tag. The protein is secreted and recoverable from the culture supernatant. The protein was purified using microfiltration, tangential ultrafiltration diafiltration, and size-exclusion chromatography (Superdex 75).Each vial of MRA-52 contains approximately 100 ?L of purified recombinant yPgs28.1VK1 in phosphate-buffered saline (pH 7.4).Protein function: antigen Tissue/cellular location: ookinete Gene coding for protein: Pgs 28 Purification methods: NI-NTA purificationQuality control methods: SDS-PAGE; mass spectrometryDuffy, P. E., P. Pimenta and D. C. Kaslow. "Pgs28 Belongs to a Family of Epidermal Growth Factor-Like Antigens that Are Targets of Malaria Transmission-Blocking Antibodies." J. Exp. Med. 177 (1993): 505-510. PubMed: 8426118.

MRA-51?? Plasmodium gallinaceum Circumsporozoite Protein (yPgCSP-RPEU/VK1 pool-1), Recombinant from Saccharomyces cerevisiae, Strain VK1(Proteins)|Plasmodium gallinaceum|Plasmodium gallinaceum Circumsporozoite Protein (yPgCSP-RPEU/VK1 pool-1), Recombinant from Saccharomyces cerevisiae, Strain VK1|-70°C|DC KaslowAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmodium gallinaceum Circumsporozoite Protein (yPgCSP-RPEU/VK1 pool-1), Recombinant from Saccharomyces cerevisiae, Strain VK1, MRA-51, contributed by David C. Kaslow."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.This reagent was authenticated by the contributor. BEI Resources has not confirmed or validated this material.Yeast-secreted recombinant circumsporozoite protein from Plasmodium gallinaceum. The open reading frame of P. gallinaceum CSP from isolate 8A expresses a recombinant protein in Saccharomyces cerevisiae, strain VK1 using the γEpRPU expression plasmid. The protein domain starts with a tyrosine, ends with a serine and has a carboxyl-terminal His6 tag. The protein is secreted and recoverable from the culture supernatant. The protein was purified using microfiltration, tangential ultrafiltration diafiltration, and size-exclusion chromatography (Superdex 75).Each vial of MRA-51 contains approximately 100 ?L of purified recombinant P. gallinaceum CSP-RPEU/VK1 in phosphate-buffered saline (pH 7.4).Protein function: antigen Tissue/cellular location: sporozoite Gene coding for protein: PgCSP Purification method: NI-NTA purificationQuality control methods: SDS-PAGE