MRA-52疟原虫28kda性阶段表面蛋白YPGS28.1,来自酿酒酵母的重组,菌株VK1(蛋白质）
产品图片
货号/SKU
MRA-52
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
MRA-52?? Plasmodium gallinaceum 28 kDa Sexual Stage Surface Protein yPgs28.1, Recombinant from Saccharomyces cerevisiae, Strain VK1(Proteins)|Plasmodium gallinaceum|Plasmodium gallinaceum 28 kDa Sexual Stage Surface Protein yPgs28.1, Recombinant from Saccharomyces cerevisiae, Strain VK1|-70°C|DC KaslowAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmodium gallinaceum 28 kDa Sexual Stage Surface Protein yPgs28.1, Recombinant from Saccharomyces cerevisiae, Strain VK1, MRA-52, contributed by David C. Kaslow."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
This reagent was authenticated by the contributor. BEI Resources has not confirmed or validated this material.
Yeast-secreted recombinant 28 kDa sexual-stage surface protein from Plasmodium gallinaceum (P. gallinaceum).The protein is expressed from the open reading frame of Pgs28 from P. gallinaceum, isolate 8A as a recombinant protein in Saccharomyces cerevisiae, strain VK1 using the pIXY154 expression plasmid. The protein expression begins at Ala22 and has a carboxyl terminal His6 tag. The protein is secreted and recoverable from the culture supernatant. The protein was purified using microfiltration, tangential ultrafiltration diafiltration, and size-exclusion chromatography (Superdex 75).
Each vial of MRA-52 contains approximately 100 ?L of purified recombinant yPgs28.1VK1 in phosphate-buffered saline (pH 7.4).
Protein function: antigen
Tissue/cellular location: ookinete
Gene coding for protein: Pgs 28
Purification methods: NI-NTA purification
Quality control methods: SDS-PAGE; mass spectrometry
Duffy, P. E., P. Pimenta and D. C. Kaslow. "Pgs28 Belongs to a Family of Epidermal Growth Factor-Like Antigens that Are Targets of Malaria Transmission-Blocking Antibodies." J. Exp. Med. 177 (1993): 505-510. PubMed: 8426118.
This reagent was authenticated by the contributor. BEI Resources has not confirmed or validated this material.
Yeast-secreted recombinant 28 kDa sexual-stage surface protein from Plasmodium gallinaceum (P. gallinaceum).The protein is expressed from the open reading frame of Pgs28 from P. gallinaceum, isolate 8A as a recombinant protein in Saccharomyces cerevisiae, strain VK1 using the pIXY154 expression plasmid. The protein expression begins at Ala22 and has a carboxyl terminal His6 tag. The protein is secreted and recoverable from the culture supernatant. The protein was purified using microfiltration, tangential ultrafiltration diafiltration, and size-exclusion chromatography (Superdex 75).
Each vial of MRA-52 contains approximately 100 ?L of purified recombinant yPgs28.1VK1 in phosphate-buffered saline (pH 7.4).
Protein function: antigen
Tissue/cellular location: ookinete
Gene coding for protein: Pgs 28
Purification methods: NI-NTA purification
Quality control methods: SDS-PAGE; mass spectrometry
Duffy, P. E., P. Pimenta and D. C. Kaslow. "Pgs28 Belongs to a Family of Epidermal Growth Factor-Like Antigens that Are Targets of Malaria Transmission-Blocking Antibodies." J. Exp. Med. 177 (1993): 505-510. PubMed: 8426118.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在这一限制下订单将在发货前发送到北欧以批准. 该试剂由贡献者认证.北京资源尚未确认或验证这种材料. 来自疟原虫（p. gallinaceum）的疟原虫重组28kDa性阶段表面蛋白（p. gallinaceum）.蛋白质是从 p的PGS28的开放阅读框中表达. Gallinaceum ，使用Pixy154表达质粒将8A作为重组蛋白，菌株VK1中的重组蛋白.蛋白质表达始于AlA22并具有羧基末端His6标签.蛋白质分泌并从培养上清液中恢复.使用微滤，切向超滤渗滤和尺寸排除色谱（Superdex 75）纯化蛋白质. 每个小瓶的MRA-52含有大约100μl纯化的重组YPGS28.1VK1在磷酸盐缓冲盐水中（pH值7.4）. -页;质谱 Duffy，P.E.，P.Pimenta和D. C. Kaslow. “PGS28属于一系列表皮生长因子样抗原，其是疟疾传输阻断抗体的靶标.” j. Exp. Med. 177（1993）：505-510. pubmed：8426118.
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