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产品图片
货号/SKU
NR-12193
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-12193?? Intimin Alpha C-Terminal Extracellular Domain from Escherichia coli O127:H6 with N-Terminal Histidine Tag, Expressed in Escherichia coli(Proteins)|Escherichia coli|Intimin Alpha C-Terminal Extracellular Domain from Escherichia coli O127:H6 with N-Terminal Histidine Tag, Expressed in Escherichia coli|-60°C or colder|AD O'Brien, JF SinclairAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Intimin Alpha C-Terminal Extracellular Domain from Escherichia coli O127:H6 with N-Terminal Histidine Tag, Expressed in Escherichia coli, NR-12193."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-12193 is a recombinant form of the C-terminal extracellular domain of the Escherichia coli (E. coli) O127:H6 outer membrane protein intimin alpha. Intimin, encoded by the eaeA gene, is an adhesin that is required for adherence of enterohemorrhagic E. coli (EHEC) to human epithelial cells and colonization of experimentally infected animals. The protein sequence includes an N-terminal hexa-histidine tag and amino acid residues of intimin alpha from EPEC O127:H6, strain E2348/69. The recombinant protein was expressed in E. coli and purified by nickel affinity chromatography. NR-12193 has a theoretical molecular weight of 42,717 daltons.
Each vial contains approximately 500 ?g of NR-12193 in PBS.
NR-12193 reacts with rabbit polyclonal antibody to E. coli intimin gamma (BEI Resources NR-12194) as shown by western blot analysis. Note: Antiserum developed against intimin gamma subtype also reacts with other intimin subtypes.
NR-12193 is a recombinant form of the C-terminal extracellular domain of the Escherichia coli (E. coli) O127:H6 outer membrane protein intimin alpha. Intimin, encoded by the eaeA gene, is an adhesin that is required for adherence of enterohemorrhagic E. coli (EHEC) to human epithelial cells and colonization of experimentally infected animals. The protein sequence includes an N-terminal hexa-histidine tag and amino acid residues of intimin alpha from EPEC O127:H6, strain E2348/69. The recombinant protein was expressed in E. coli and purified by nickel affinity chromatography. NR-12193 has a theoretical molecular weight of 42,717 daltons.
Each vial contains approximately 500 ?g of NR-12193 in PBS.
NR-12193 reacts with rabbit polyclonal antibody to E. coli intimin gamma (BEI Resources NR-12194) as shown by western blot analysis. Note: Antiserum developed against intimin gamma subtype also reacts with other intimin subtypes.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. NR-12193是C末端细胞外结构域的重组形式的大肠杆菌( e.COLI )O127:H6外膜蛋白质内含物α.由 eae 的基因编码的内菊酯是肠溶性 en粘附所需的粘附素. COLI (EHEC)对人上皮细胞和实验感染动物的定植.蛋白质序列包括来自EPEC O127:H6,菌株E2348 / 69的内膜α的N-末端六甲基 - 组氨酸标签和氨基酸残基.重组蛋白在 e中表达. COLI 通过镍亲和层析纯化. NR-12193具有42,717道尔顿的理论分子量. 每个小瓶在PBS中含有大约500μg的NR-12193. NR-12193与兔多克隆抗体反应 e . Coli 内含γ伽马(BEI资源NR-12194),如Western印迹分析所示.注意:针对内氨酰γ亚型开发的抗血清也与其他内含物亚型反应.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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