- 4 次围观
产品图片
货号/SKU
NR-14832
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-14832??Mycobacterium tuberculosis, CDC1551, Cell Membrane Fraction(Antigen Preparations)|Mycobacterium tuberculosis|CDC1551, Cell Membrane Fraction|-80°C or colder|BEI Resources or NIH - TB Vaccine Testing and Research Materials ContractAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain CDC1551, Cell Membrane Fraction, NR-14832."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-14832 is a preparation of the cell membrane fraction of Mycobacterium tuberculosis, strain CDC1551, and contains the cytoplasmic membrane and components of the outer lipid layer.
The culture was grown to late log phase in glycerol-alanine-salts medium, washed with PBS and inactivated by gamma irradiation. The bacilli were broken in a French Press pressure cell and unbroken cells were removed by low speed centrifugation. The cell wall was isolated by centrifugation at 27,000 × g. The supernatant was subjected to a 100,000 × g centrifugation for four hours and the resulting membrane pellet was washed with PBS, then suspended and dialyzed in 10 mM ammonium bicarbonate. Protein content was determined using the BCA protein assay.
Each vial contains approximately 1 mg of cell membrane fraction from M. tuberculosis, strain CDC1551 provided in 10 mM ammonium bicarbonate.
NR-14832 is a preparation of the cell membrane fraction of Mycobacterium tuberculosis, strain CDC1551, and contains the cytoplasmic membrane and components of the outer lipid layer.
The culture was grown to late log phase in glycerol-alanine-salts medium, washed with PBS and inactivated by gamma irradiation. The bacilli were broken in a French Press pressure cell and unbroken cells were removed by low speed centrifugation. The cell wall was isolated by centrifugation at 27,000 × g. The supernatant was subjected to a 100,000 × g centrifugation for four hours and the resulting membrane pellet was washed with PBS, then suspended and dialyzed in 10 mM ammonium bicarbonate. Protein content was determined using the BCA protein assay.
Each vial contains approximately 1 mg of cell membrane fraction from M. tuberculosis, strain CDC1551 provided in 10 mM ammonium bicarbonate.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
NR-14832是一种制剂的膜膜分数的细胞膜分枝杆菌 - 菌分CDC1551，含有细胞质膜和外脂层的组分.
将培养物生长至甘油 - 丙氨酸 - 盐培养基中的晚记物相，用PBS洗涤并通过γ辐射灭活.杆菌在法国压力机中被破坏，通过低速离心除去了不间断的细胞.通过以27,000×g离心分离细胞壁.将上清液经过100,000×G离心4小时，用PBS洗涤所得膜沉淀，然后悬浮并在10mM碳酸氢铵中透析.使用BCA蛋白质测定法测定蛋白质含量.
每个小瓶含有大约1mg的 m的细胞膜馏分.结核病，菌株CDC1551提供10mM碳酸氢铵.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
品牌标识
可能感兴趣的内容
2022-04-01
2022-04-01
2021-12-21
2024-09-22
2022-04-01
2024-10-20
2021-12-21
2022-03-31
2024-05-19
2022-04-01
2021-12-13
2024-06-25
2022-04-01