A/turkey/Wisconsin/1/1966 (H9N2)

NR-43783?? N2 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/turkey/Wisconsin/1/1966 (H9N2), Recombinant from Baculovirus(Proteins)|Influenza A virus|N2 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/turkey/Wisconsin/1/1966 (H9N2), Recombinant from Baculovirus|-20°C or colder|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: N2 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/turkey/Wisconsin/1/1966 (H9N2), Recombinant from Baculovirus, NR-43783."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.A recombinant form of the N2 neuraminidase (NA) protein from influenza A virus, A/turkey/Wisconsin/1/1966 (H9N2) containing an N-terminal histidine tag was produced in Sf9 insect cells using a baculovirus expression vector system and purified by nickel affinity chromatography. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal eight-histidine tag followed by a 43 amino acid tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site, as described for the 1918 pandemic virus. The full-length NA precursor protein is 469 residues (GenPept: CAE00569). Each vial contains approximately 200 ?g of purified recombinant NA protein in PBS (pH 7.4).NR-43783 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2'-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (4-MUNANA).

NR-43782?? H9 Hemagglutinin (HA) Protein with C-Terminal Histidine Tag from Influenza Virus, A/turkey/Wisconsin/1/1966 (H9N2), Recombinant from Baculovirus(Proteins)|Influenza A virus|H9 Hemagglutinin (HA) Protein with C-Terminal Histidine Tag from Influenza Virus, A/turkey/Wisconsin/1/1966 (H9N2), Recombinant from Baculovirus|4°C|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: H9 Hemagglutinin (HA) Protein with C-Terminal Histidine Tag from Influenza Virus, A/turkey/Wisconsin/1/1966 (H9N2), Recombinant from Baculovirus, NR-43782."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.A recombinant form of the H9 hemagglutinin (HA) protein from influenza A virus, A/turkey/Wisconsin/1/1966 (H9N2) containing a C-terminal histidine tag was produced in Sf9 insect cells using a baculovirus expression vector system and was purified by nickel affinity chromatography. The HA protein includes a C-terminal peptide containing a thrombin cleavage site, trimerizing (foldon) domain and eight histidine residues. The full-length HA precursor protein is 560 residues (GenPept: AGB50938).Each vial contains approximately 100 ?g to 200 ?g of purified recombinant HA protein in PBS (pH 7.4) with 1mM 4-(2-aminoethyl)-benzenesulfonyl fluoride hydrochloride (AEBSF), and 0.5M NaCl.