A/chicken/Netherlands/14015531/2014 (H5N8)

NR-50134?? N8 Neuraminidase (NA) Protein from Influenza Virus, A/chicken/Netherlands/14015531/2014 (H5N8), Recombinant from Baculovirus(Proteins)|Influenza A virus|N8 Neuraminidase (NA) Protein from Influenza Virus, A/chicken/Netherlands/14015531/2014 (H5N8), Recombinant from Baculovirus|-20°C|P Palese, F Krammer, R HaiAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: N8 Neuraminidase (NA) Protein from Influenza Virus, A/chicken/Netherlands/14015531/2014 (H5N8), Recombinant from Baculovirus, NR-50134."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.A recombinant form of the N8 neuraminidase (NA) protein from influenza A virus, A/chicken/Netherlands/14015531/2014 (H5N8) was produced in Sf9 insect cells using a baculovirus expression vector system. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal six histidine tag followed by a tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site. The protein was purified by nickel affinity chromatography and then treated with thrombin to remove the tetramerization domain and the histidine tag. The thrombin-treated protein was further purified prior to final formulation. The full-length NA precursor protein is 470 residues (GISAID EpiFlu: EPI548626). Each vial contains 50 ?g to 100 ?g of purified recombinant NA protein in PBS (pH 7.4) with 50% glycerol. NR-50134 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2’-(4-methylumbelliferyl)-a-d-N-acetylneuraminic acid (4-MUNANA).   

NR-50111?? N8 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/chicken/Netherlands/14015531/2014 (H5N8), Recombinant from Baculovirus(Proteins)|Influenza A virus|N8 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/chicken/Netherlands/14015531/2014 (H5N8), Recombinant from Baculovirus|-20°C|F KrammerAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: N8 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/chicken/Netherlands/14015531/2014 (H5N8), Recombinant from Baculovirus, NR-50111."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.A recombinant form of the N8 Neuraminidase (NA) protein from influenza A virus, A/chicken/Netherlands/14015531/2014 (H5N8) containing an N-terminal histidine tag was produced in Sf9 insect cells using a baculovirus expression vector system and was purified by nickel affinity chromatography. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal six histidine tag followed by a tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site. The full-length NA precursor protein is 470 residues (GISAID EpiFlu: EPI548626).Each vial contains 50 ?g to 150 ?g of purified recombinant NA protein in PBS (pH 7.4) with 50% glycerol. NR-50111 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2'-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (4-MUNANA).

NR-50110?? H5 Hemagglutinin (HA) Protein with C-Terminal Histidine Tag from Influenza Virus, A/chicken/Netherlands/14015531/2014 (H5N8), Recombinant from Baculovirus(Proteins)|Influenza A virus|H5 Hemagglutinin (HA) Protein with C-Terminal Histidine Tag from Influenza Virus, A/chicken/Netherlands/14015531/2014 (H5N8), Recombinant from Baculovirus|-20°C|F KrammerAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: H5 Hemagglutinin (HA) Protein with C-Terminal Histidine Tag from Influenza Virus, A/chicken/Netherlands/14015531/2014 (H5N8), Recombinant from Baculovirus, NR-50110."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.A recombinant form of the H5 hemagglutinin (HA) protein from influenza A virus, A/chicken/Netherlands/14015531/2014 (H5N8) containing a C-terminal histidine tag was produced in Sf9 insect cells using a baculovirus expression vector system and purified by nickel affinity chromatography. The predicted transmembrane and endodomain coding regions of the HA gene were replaced with a synthetic gene segment encoding a thrombin cleavage site, trimerizing (foldon) domain and six histidine residues, as described for several influenza A virus subtypes. The full-length HA precursor protein is 567 residues (GISAID EpiFlu: EPI548623).Each vial contains 50 ?g to 150 ?g of purified recombinant HA protein in PBS (pH 7.4).