NR-50134来自流感病毒的N8神经氨酸酶(NA)蛋白,A/chicken/Netherlands/14015531/2014(H5N8),来自杆状病毒的重组蛋白(蛋白质）
产品图片
货号/SKU
NR-50134
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-50134?? N8 Neuraminidase (NA) Protein from Influenza Virus, A/chicken/Netherlands/14015531/2014 (H5N8), Recombinant from Baculovirus(Proteins)|Influenza A virus|N8 Neuraminidase (NA) Protein from Influenza Virus, A/chicken/Netherlands/14015531/2014 (H5N8), Recombinant from Baculovirus|-20°C|P Palese, F Krammer, R HaiAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: N8 Neuraminidase (NA) Protein from Influenza Virus, A/chicken/Netherlands/14015531/2014 (H5N8), Recombinant from Baculovirus, NR-50134."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the N8 neuraminidase (NA) protein from influenza A virus, A/chicken/Netherlands/14015531/2014 (H5N8) was produced in Sf9 insect cells using a baculovirus expression vector system. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal six histidine tag followed by a tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site. The protein was purified by nickel affinity chromatography and then treated with thrombin to remove the tetramerization domain and the histidine tag. The thrombin-treated protein was further purified prior to final formulation. The full-length NA precursor protein is 470 residues (GISAID EpiFlu: EPI548626).
Each vial contains 50 ?g to 100 ?g of purified recombinant NA protein in PBS (pH 7.4) with 50% glycerol.
A recombinant form of the N8 neuraminidase (NA) protein from influenza A virus, A/chicken/Netherlands/14015531/2014 (H5N8) was produced in Sf9 insect cells using a baculovirus expression vector system. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal six histidine tag followed by a tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site. The protein was purified by nickel affinity chromatography and then treated with thrombin to remove the tetramerization domain and the histidine tag. The thrombin-treated protein was further purified prior to final formulation. The full-length NA precursor protein is 470 residues (GISAID EpiFlu: EPI548626).
Each vial contains 50 ?g to 100 ?g of purified recombinant NA protein in PBS (pH 7.4) with 50% glycerol.
NR-50134 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2’-(4-methylumbelliferyl)-a-d-N-acetylneuraminic acid (4-MUNANA).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.该限制的订单将在发货前发送到NIAID.使用杆状病毒表达载体系统在SF9昆虫细胞中产生H5N8.将Na基因的预测的外胚瘤编码区域融合到编码N-末端六个组氨酸标签的合成基因区段，然后由血管扩张剂刺激的磷蛋白（VASP）和凝血酶切割位点为四聚化结构域.通过镍亲和色谱法纯化蛋白质，然后用??凝血酶处理以除去四聚化结构域和组氨酸标签.在最终制剂之前进一步纯化凝血酶处理的蛋白质.全长Na前体蛋白是470个残基（GisaID epiflu：EPI548626）. 每个小瓶含有50μg至100μg的PBS（pH7.4）中的纯化重组Na蛋白，其中50％甘油. nr-50134基于其切割荧光底物2' - （4-甲基纤维素）-adn-乙酰甘氨酸（4-munana）的能力，证明功能敏活性. ＆nbsp;＆nbsp;
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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