产品描述信息
NR-43784?? N2 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Brisbane/10/2007 (H3N2), Recombinant from Baculovirus(Proteins)|Influenza A virus|N2 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Brisbane/10/2007 (H3N2), Recombinant from Baculovirus|-20°C or colder|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: N2 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Brisbane/10/2007 (H3N2), Recombinant from Baculovirus, NR-43784."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.A recombinant form of the N2 neuraminidase (NA) protein from influenza A virus, A/Brisbane/10/2007 (H3N2) containing an N-terminal histidine tag was produced in Sf9 insect cells using a baculovirus expression vector system and purified by nickel affinity chromatography. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal eight-histidine tag followed by a 43 amino acid tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site, as described for the 1918 pandemic virus. The full-length NA precursor protein is 469 residues (GenPept: AFN11835). Each vial contains approximately 200 ?g of purified recombinant NA protein in D-PBS (pH 7.4).NR-43784 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2’-(4-methylumbelliferyl)-a-d-N-acetylneuraminic acid (4-MUNANA).