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产品图片
货号/SKU
NR-50173
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-50173?? N7 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/harbor seal/Germany/1/2014 (H10N7), Recombinant from Baculovirus(Proteins)|Influenza A virus|N7 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/harbor seal/Germany/1/2014 (H10N7), Recombinant from Baculovirus|-20°C or colder|F KrammerAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: N7 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/harbor seal/Germany/1/2014 (H10N7), Recombinant from Baculovirus, NR-50173.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the N7 neuraminidase (NA) protein from influenza A virus, A/harbor seal/Germany/1/2014 (H10N7) containing an N-terminal histidine tag was produced in Sf9 insect cells using a baculovirus expression vector system and was purified by nickel affinity chromatography. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal six histidine tag followed by a tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site.
Each vial contains 50 ?g to 100 ?g of purified recombinant NA protein in 50 mM Na2HPO4, pH 8, with 500 mM NaCl and 5 mM EDTA.
NR-50173 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2’-(4-methylumbelliferyl)-a-D-N-acetylneuraminic acid (4-MUNANA).
A recombinant form of the N7 neuraminidase (NA) protein from influenza A virus, A/harbor seal/Germany/1/2014 (H10N7) containing an N-terminal histidine tag was produced in Sf9 insect cells using a baculovirus expression vector system and was purified by nickel affinity chromatography. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal six histidine tag followed by a tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site.
Each vial contains 50 ?g to 100 ?g of purified recombinant NA protein in 50 mM Na2HPO4, pH 8, with 500 mM NaCl and 5 mM EDTA.
NR-50173 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2’-(4-methylumbelliferyl)-a-D-N-acetylneuraminic acid (4-MUNANA).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. N7神经氨酸酶的重组形式(NA)
来自流感的蛋白质,a / harbour封印/德国/174(h10n7) 含有n末端组氨酸标签,在SF9昆虫细胞中使用a制备
Baculovirus表达载体系统并通过镍亲和力纯化
色谱法.预测的ectodomain
Na基因的编码区域与编码的合成基因段融合
N-末端六个组氨酸标签,然后是四聚化结构域
血管扩张剂刺激的磷蛋白(VASP)和凝血酶切割位点.
小瓶含有50μg至100μg纯化的重组Na蛋白在50mM Na 2 HPO 4 中,
pH 8,用500mM NaCl和5mM EDTA. NR-50173被证明在功能上有效
基于其切割荧光基材的能力
2' - (4-甲基苞片)-a-d-n-acetylneuraminic
酸(4-munana).
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
品牌标识
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