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产品图片
货号/SKU
NR-43781
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-43781?? Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, B/Hong Kong/330/2001 (Victoria Lineage), Recombinant from Baculovirus(Proteins)|Influenza B virus|Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, B/Hong Kong/330/2001 (Victoria Lineage), Recombinant from Baculovirus|-80°C or colder|BEI ResourcesAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, B/Hong Kong/330/2001 (Victoria Lineage), Recombinant from Baculovirus, NR-43781."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval.
This item is currently in our production queue. Please allow ample time for distribution lots to be made available.
A recombinant form of the neuraminidase (NA) protein from influenza B virus, B/Hong Kong/330/2001 (Victoria Lineage) containing an N-terminal histidine tag was produced in Sf9 insect cells using a baculovirus expression vector system and purified by nickel affinity chromatography. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal eight-histidine tag followed by a 43 amino acid tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site, as described for the 1918 pandemic virus. The full-length NA precursor protein is 466 residues (GenPept: AA038878).
Each vial contains 20 ?g to 80 ?g of purified recombinant NA protein in 50 mM Tris (pH 8.0) with 500 mM NaCl.
NR-43781 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2’-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (4-MUNANA).
This item is currently in our production queue. Please allow ample time for distribution lots to be made available.
A recombinant form of the neuraminidase (NA) protein from influenza B virus, B/Hong Kong/330/2001 (Victoria Lineage) containing an N-terminal histidine tag was produced in Sf9 insect cells using a baculovirus expression vector system and purified by nickel affinity chromatography. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal eight-histidine tag followed by a 43 amino acid tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site, as described for the 1918 pandemic virus. The full-length NA precursor protein is 466 residues (GenPept: AA038878).
Each vial contains 20 ?g to 80 ?g of purified recombinant NA protein in 50 mM Tris (pH 8.0) with 500 mM NaCl.
NR-43781 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2’-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (4-MUNANA).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将被发送到北欧以获得批准.
此项目目前处于我们的生产队列中.请允许充足的时间进行分销批次.
使用杆状病毒表达载体系统在SF9昆虫细胞中产生来自流感B病毒,B /香港/ 330/2001(维多利亚谱系)的神经氨酸酶(Na)蛋白的重组形式,B /香港/ 330/2001(维多利亚谱系)在SF9昆虫细胞中产生并通过镍纯化亲和层析.将NA基因的预测的外胚层编码区域融合到编码N-末端8-组氨酸标签的合成基因区段,然后由血管扩张剂刺激的磷蛋白(VASP)和凝血酶切割位点为43个氨基酸四聚化结构域,如上所述1918年的大流行病病毒.全长Na前体蛋白是466个残基(Genpept:AA038878).
每个小瓶含有20μg至80μg的纯化重组Na蛋白,50mM Tris(pH8.0),500mM NaCl.
基于其切割荧光底物2' - (4-甲基蛋糖)-α-D-N-乙酰氨酰氨基酸(4-MUNANA)的能力,证明NR-43781在功能上活性活性活性.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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