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产品图片
货号/SKU
NR-44364
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-44364?? N9 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Shanghai/1/2013 (H7N9), Recombinant from Baculovirus(Proteins)|Influenza A virus|N9 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Shanghai/1/2013 (H7N9), Recombinant from Baculovirus|2°C to 8°C|P Palese, F Krammer, R HaiAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: N9 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Shanghai/1/2013 (H7N9), Recombinant from Baculovirus, NR-44364."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the N9 neuraminidase (NA) protein from influenza A virus, A/Shanghai/1/2013 (H7N9) containing an N-terminal histidine tag was produced in Sf9 insect cells using a baculovirus expression vector system and purified by nickel affinity chromatography. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal six histidine tag followed by a tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site. The full-length NA precursor protein is 465 residues (GISAID EpiFlu: EPI439487).
Each vial contains 25 ?g to 75 ?g of purified recombinant NA protein in 50 mM Tris (pH 8.0), 500 mM NaCl, and 0.2% sodium azide.
NR-44364 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2'-(4-methylumbelliferyl)-a-D-N-acetylneuraminic acid (4-MUNANA).
A recombinant form of the N9 neuraminidase (NA) protein from influenza A virus, A/Shanghai/1/2013 (H7N9) containing an N-terminal histidine tag was produced in Sf9 insect cells using a baculovirus expression vector system and purified by nickel affinity chromatography. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal six histidine tag followed by a tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site. The full-length NA precursor protein is 465 residues (GISAID EpiFlu: EPI439487).
Each vial contains 25 ?g to 75 ?g of purified recombinant NA protein in 50 mM Tris (pH 8.0), 500 mM NaCl, and 0.2% sodium azide.
NR-44364 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2'-(4-methylumbelliferyl)-a-D-N-acetylneuraminic acid (4-MUNANA).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. 来自甲型流感病毒的N9神经氨酸酶(NA)蛋白的重组形式,A / Shangah / 1/2013(H7N9)使用杆状病毒表达载体系统在SF9昆虫细胞中产生含有N-末端组氨酸标签,并通过镍亲和层析纯化.将Na基因的预测的外胚瘤编码区域融合到编码N-末端六个组氨酸标签的合成基因区段,然后由血管扩张剂刺激的磷蛋白(VASP)和凝血酶切割位点为四聚化结构域.全长Na前体蛋白是465个残基(GisaID epiflu:EPI439487). 每个小瓶含有25μg至75μg纯化的重组Na蛋白,50mM Tris(pH8.0),500mM NaCl,和0.2%叠氮化钠. NR-44364基于其切割荧光底物2' - (4-甲基纤维素)-ADN-乙酰氨基丙氨酸(4-MUNANA)的能力来表现为功能源性活性.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
品牌标识
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