NR-50349?? Escherichia coli - Staphylococcus aureus Shuttle Vector pKK30, Recombinant in Escherichia coli(Plasmid/Vectors)|Escherichia coli|Escherichia coli - Staphylococcus aureus Shuttle Vector pKK30, Recombinant in Escherichia coli|-60°C or colder|JL Bose, EV StabbAcknowledgment for publications should read “The following reagent was contributed by Dr. J. L. Bose for distribution by BEI Resources, NIAID, NIH: Escherichia coli – Staphylococcus aureus Shuttle Vector pKK30, Recombinant in Escherichia coli, NR-50349.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. NR-50349 is a preserved
culture of
Escherichia
coli (
E. coli)
DH5αλpir containing the
E. coli-staphylococcal shuttle vector pKK30. Vector pKK30 contains the
E. coli R6Kγ origin of replication and
is for use in
Staphylococcus spp. strains without LAC-p01. Vector pKK30 contains a single trimethoprim resistance cassette that is functional in both
E. coli and
Staphylococcus spp. pKK30 is identical to pKK22 except it lacks open reading frames required by USA300 strains needed for plasmid maintenance. The complete pKK30 nucleotide sequence is available (GenBank:
KX085043) and the vector map of pKK30 is available on the Product Information Sheet. pKK30 was deposited in conjunction with pKK22 and
E.
coli strains DH5αλpir and GM2163λpir (available as a kit, BEI Resources
NR-50352). pKK22 and
pKK30 were created to maintain stability in
E. coli and
Staphylococcus species without antibiotic selection
during
in vitro and
in vivo experiments. The
E. coli R6Kγ origin of replication of
both vectors requires
pir+ for replication which is provided in either
DH5αλpir or GM2163λpir
E.
coli strains.
Catalog Number |
Vector or Host |
Comments |
NR-50348 |
pKK22 |
For use in E. coli DH5αλpir or GM2163λpir or S. sureus USA300 strains containing LAC-p01 |
NR-50349 |
pKK30 |
pKK30 is a variant of pKK22, for use in E. coli DH5αλpir or GM2163λpir or Staphylococcus species not containing LAC-p01 |
NR-50350 |
E. coli DH5αλpir |
Host strain containing the pir genes for performing genetic manipulations prior to transfer into Staphylococcus (F-?80dlacZ ΔM15 ΔacZYA-argF U169 deoR supE44 hsdR17 recA1 endA1 gyrA96 thi-1 relA1) |
NR-50351 |
E. coli GM2163λpir |
Host strain containing the pir genes for performing genetic manipulations. This strain is also a dam and dcm methylase mutant for transfer of plasmids into Staphylococcus isolates that do not accept E. coli DNA easily (F-ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 rpsL136 dam13::Tn9 xylA5 mtl-1 thi-1 mcrB1 hsdR2 λpir) |
This strain was developed by Krute, et al., at the Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, PubMed:
27637878.Each vial of NR-50349 contains
approximately 0.5 mL of
E. coli,
DH5αλpir, in Tryptic Soy broth
containing 10 ?g/mL trimethoprim supplemented with 10% glycerol.