NR-41869表达载体,含有来自光滑生物指肠的热休克蛋白70基因片段,在大肠杆菌中重组(质粒/载体)
产品图片
货号/SKU
NR-41869
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-41869?? Expression Vector Containing the Heat Shock Protein 70 Gene Fragment from Biomphalaria glabrata, Recombinant in Escherichia coli(Plasmid/Vectors)|Biomphalaria glabrata|Expression Vector Containing the Heat Shock Protein 70 Gene Fragment from Biomphalaria glabrata, Recombinant in Escherichia coli|-80°C or colder|M TuckerAcknowledgment for publications should read "The following reagent was provided by the NIAID Schistosomiasis Resource Center for distribution through BEI Resources, NIAID, NIH: Expression Vector Containing the Heat Shock Protein 70 Gene Fragment from Biomphalaria glabrata, Recombinant in Escherichia coli, NR-41869."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval.
NR-41869 is an expression vector (pCR?2.1-TOPO?), containing a fragment of the heat shock protein (HSP) 70 gene from Biomphalaria glabrata (B. glabrata), transformed into Escherichia coli (E. coli), strain DH5a.
To create the insert, RNA was extracted from juvenile B. glabrata, strain NMRI, that were exposed to Schistosoma mansoni. First strand cDNA was synthesized with oligo-poly(dT)15. To construct the library, first strand cDNA was processed into all steps of suppression subtractive hybridization (SSH) using the Clontech? PCR-Select? cDNA Subtraction Kit. PCR was performed using adaptor-specific primers for the HSP 70 gene fragment. The amplified product was cloned into pCR?2.1-TOPO? (Invitrogen? Life Technologies?) cloning vector, and was transformed into E. coli, strain DH5a.
The expressed sequence tag (EST) of the HSP 70 gene fragment is available (GenBank: GH717081).
Each vial contains approximately 1 mL of E. coli, strain DH5a, in Luria Bertani (LB) broth containing 100 ?g/mL of ampicillin supplemented with 15% glycerol.
NR-41869 is an expression vector (pCR?2.1-TOPO?), containing a fragment of the heat shock protein (HSP) 70 gene from Biomphalaria glabrata (B. glabrata), transformed into Escherichia coli (E. coli), strain DH5a.
To create the insert, RNA was extracted from juvenile B. glabrata, strain NMRI, that were exposed to Schistosoma mansoni. First strand cDNA was synthesized with oligo-poly(dT)15. To construct the library, first strand cDNA was processed into all steps of suppression subtractive hybridization (SSH) using the Clontech? PCR-Select? cDNA Subtraction Kit. PCR was performed using adaptor-specific primers for the HSP 70 gene fragment. The amplified product was cloned into pCR?2.1-TOPO? (Invitrogen? Life Technologies?) cloning vector, and was transformed into E. coli, strain DH5a.
The expressed sequence tag (EST) of the HSP 70 gene fragment is available (GenBank: GH717081).
Each vial contains approximately 1 mL of E. coli, strain DH5a, in Luria Bertani (LB) broth containing 100 ?g/mL of ampicillin supplemented with 15% glycerol.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将被发送到NIAID批准. NR-41869是表达式向量( PCR ? 2.1-topo ? ),含有来自生物骨髓菌的热休克蛋白(Hsp)70基因的片段( B.Glabrata)(转化为大肠杆菌(大肠杆菌),菌株DH5A. 从青少年 b中提取RNA. Glabrata ,菌株NMRI,其暴露于血吸虫麦森(Schistosoma Mansoni).用寡聚聚(DT)15合成第一链cDNA.为了构建文库,使用CLONTECH ? PCR-SELECT TM cDNA减法试剂盒将第一链cDNA加工成抑制减去杂交(SSH)的所有步骤.使用针对HSP 70基因片段的适配器特异性引物进行PCR.将扩增的产物克隆到PCR ? 2.1-topo ?(Invitrogen?Life Technologies?)克隆载体中,并转化为 e. COLI ,菌株DH5A. HSP 70基因片段的表达序列标签(EST)可用(GenBank: gh717081 ). 每个小瓶包含大约1毫升 e. Coli ,菌株DH5a,在Luria Bertani(LB)肉汤中,含有100μg/ ml补充有15%甘油的氨苄青霉素.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
品牌标识
可能感兴趣的内容
2022-04-01
2022-04-01
2022-08-11
2021-12-21
2021-12-13
2022-06-17
2022-01-10
2022-06-17
2022-04-01
2022-04-01
2024-01-21
2022-03-31