NR-50349大肠杆菌-金黄色葡萄球菌穿梭载体pKK30,在大肠杆菌中重组(质粒/载体）

NR-50349?? Escherichia coli - Staphylococcus aureus Shuttle Vector pKK30, Recombinant in Escherichia coli(Plasmid/Vectors)|Escherichia coli|Escherichia coli - Staphylococcus aureus Shuttle Vector pKK30, Recombinant in Escherichia coli|-60°C or colder|JL Bose, EV StabbAcknowledgment for publications should read “The following reagent was contributed by Dr. J. L. Bose for distribution by BEI Resources, NIAID, NIH: Escherichia coli – Staphylococcus aureus Shuttle Vector pKK30, Recombinant in Escherichia coli, NR-50349.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. NR-50349 is a preserved culture of Escherichia coli (E. coli) DH5αλpir containing the E. coli-staphylococcal shuttle vector pKK30. Vector pKK30 contains the E. coli R6Kγ origin of replication and is for use in Staphylococcus spp. strains without LAC-p01. Vector pKK30 contains a single trimethoprim resistance cassette that is functional in both E. coli and Staphylococcus spp. pKK30 is identical to pKK22 except it lacks open reading frames required by USA300 strains needed for plasmid maintenance.  The complete pKK30 nucleotide sequence is available (GenBank: KX085043) and the vector map of pKK30 is available on the Product Information Sheet. pKK30 was deposited in conjunction with pKK22 and E. coli strains DH5αλpir and GM2163λpir (available as a kit, BEI Resources NR-50352). pKK22 and pKK30 were created to maintain stability in E. coli and Staphylococcus species without antibiotic selection during in vitro and in vivo experiments. The E. coli R6Kγ origin of replication of both vectors requires pir+ for replication which is provided in either DH5αλpir or GM2163λpir E. coli strains.

Catalog Number	Vector or Host	Comments
NR-50348	pKK22	For use in E. coli DH5αλpir or GM2163λpir or S. sureus USA300 strains containing LAC-p01
NR-50349	pKK30	pKK30 is a variant of pKK22, for use in E. coli DH5αλpir or GM2163λpir or Staphylococcus species not containing LAC-p01
NR-50350	E. coli DH5αλpir	Host strain containing the pir genes for performing genetic manipulations prior to transfer into Staphylococcus (F-?80dlacZ ΔM15 ΔacZYA-argF U169 deoR supE44 hsdR17 recA1 endA1 gyrA96 thi-1 relA1)
NR-50351	E. coli GM2163λpir	Host strain containing the pir genes for performing genetic manipulations. This strain is also a dam and dcm methylase mutant for transfer of plasmids into Staphylococcus isolates that do not accept E. coli DNA easily (F-ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 rpsL136 dam13::Tn9 xylA5 mtl-1 thi-1 mcrB1 hsdR2 λpir)

This strain was developed by Krute, et al., at the Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, PubMed:  27637878.Each vial of NR-50349 contains approximately 0.5 mL of E. coli, DH5αλpir, in Tryptic Soy broth containing 10 ?g/mL trimethoprim supplemented with 10% glycerol.