来自杆状病毒(蛋白质）的重组，NR-1_Vaccinia virus

产品基础信息

生物安全等级建议分类：美国、1

产品描述信息

NR-52308?? Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from Baculovirus(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from Baculovirus| -60°C or colder|F Krammer, F Amanat, S StrohmeierAcknowledgment for publications should read “The following reagent was produced under HHSN272201400008C and obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from Baculovirus, NR-52308.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A recombinant form of the spike (S) glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QJE37812) was produced by transfection of purified plasmid in SF9 insect cells using a baculovirus expression system and purified by nickel affinity chromatography. NR-52308 lacks the signal sequence and contains 1196 residues (ectodomain) of the SARS-CoV-2 spike glycoprotein; the recombinant protein was modified to remove the polybasic S1/S2 cleavage site (RRAR to A; residues 682 to 685), stabilized with a pair of mutations (K986P and V987P, wild type numbering; GenPept: QHD43416) and includes a thrombin cleavage site, T4 foldon trimerization domain and C-terminal hexa-histidine tag. NR-52308 has a theoretical molecular weight of 137,600 daltons. The crystal structure for trimeric S glycoprotein from SARS-CoV-2 has been solved at 3.20 angstrom resolution (PDB: 6VYB). Note: For a detailed protocol and list of related items, see https://labs.icahn.mssm.edu/krammerlab/covid-19/ The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor, allows fusion of the viral and cellular membranes. The S protein is a target for neutralizing antibodies. NR-52308 reacts with monoclonal anti-histidine tag in western blot analysis. NR-52308 is intended for western blot, ELISA and animal vaccination. Each vial of NR-52308 contains purified recombinant protein in phosphate buffered saline (PBS). Note: The long-term stability of this preparation is not known. It is recommended that users confirm the activity of the product if not used within three months of receipt. Additional information and tools are available at ViPR (Virus Pathogen Resource).

主要内容

此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准. 来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2），武汉-U-1（Genpept： qhd43416 ）并且包括凝血酶切割位点，T4 folcon三聚化结构域和c末端六核分裂标签. NR-52308具有137,600道尔顿的理论分子量.来自SARS-COV-2的三聚体S糖蛋白的晶体结构已在3.20埃左右求解分辨率（PDB： 6vyb ）. 注意：有关相关项的详细协议和列表，请参阅https://labs.icahn.mssm.edu/krammerlab/covid-19/ S糖蛋白介导与宿主血管紧张素转化酶2（ACE2）的病毒结合.该蛋白质形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜. S蛋白是中和抗体的靶标. NR-52308与蛋白质印迹分析中的单克隆抗组氨酸标签反应. NR-52308适用于Western Blot，ELISA和动物疫苗接种. 每个小瓶NR-52308含有磷酸盐缓冲盐水（PBS）的纯化的重组蛋白. 注意：该准备的长期稳定性是未知的.建议用户如果不在收到的三个月内使用，请确认产品的活动. vipr （病毒病原体资源）提供了附加信息和工具.

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BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。

除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。

BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。

品牌标识

产品关键词

NR-52308 来自 SARS 相关冠状病毒 2 的刺突糖蛋白(稳定）

Wuhan-Hu-1 带有 C 末端组氨酸标签

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NR-49734带有C末端组氨酸标签的H3血凝素(HA)蛋白来自流感病毒,A/Perth/16/2009(H3N2),来自杆状病毒(蛋白质）的重组

NR-49734_ H3 Hemagglutinin (HA) Protein with C-Terminal Histidine Tag from Influenza Virus, A/Perth/16/2009 (H3N2), Recombinant from Baculovirus(Proteins)

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产品英文名称

产品别名

NR-49734_ H3 Hemagglutinin (HA) Protein with C-Terminal Histidine Tag from Influenza Virus, A/Perth/16/2009 (H3N2), Recombinant from Baculovirus(Proteins)
NR-49734 带有 C 末端组氨酸标签的 H3 血凝素 (HA) 蛋白来自流感病毒，A/Perth/16/2009 (H3N2)，来自杆状病毒（蛋白质）的重组

货号/SKU

NR-49734

货号/规格

库存与交货期

3-8周

人民币价格

14000

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本商品人民币2024年销售价格正在调整中，请等待更新完毕。

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BEI Resources(ATCC)

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产品基础信息

生物安全等级建议分类：美国、1

产品描述信息

NR-49734?? H3 Hemagglutinin (HA) Protein with C-Terminal Histidine Tag from Influenza Virus, A/Perth/16/2009 (H3N2), Recombinant from Baculovirus(Proteins)|Influenza A virus|H3 Hemagglutinin (HA) Protein with C-Terminal Histidine Tag from Influenza Virus, A/Perth/16/2009 (H3N2), Recombinant from Baculovirus|-80°C|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: H3 Hemagglutinin (HA) Protein with C-Terminal Histidine Tag from Influenza Virus, A/Perth/16/2009 (H3N2), Recombinant from Baculovirus, NR-49734."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A recombinant form of the H3 hemagglutinin (HA) protein from influenza A virus, A/Perth/16/2009 (H3N2) containing a C-terminal octa-histidine tag was produced in Sf9 insect cells using a baculovirus expression vector system and was purified by nickel affinity chromatography. The HA protein includes a C-terminal peptide containing a thrombin cleavage site, trimerizing (foldon) domain and eight histidine residues. The full-length HA precursor protein is 566 residues (GenPept: ACS71642). NR-49734 has a theoretical molecular weight of 62,300 daltons. NR-49734 does not exhibit hemagglutination activity. Each vial contains 100 ?g to 200 ?g of NR-49734 in 50 mM Tris-HCl (pH 8) with 100 mM NaCl and 15% glycerol.

主要内容

此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准. 使用杆状病毒表达载体系统在SF9昆虫细胞中产生来自流感的H3血凝素（HA）蛋白的H3血凝素（HA）蛋白的重组形式，含有C-末端Octa-组氨酸标签，并纯化通过镍亲和层析.哈哈蛋白质包括含有凝血酶裂解位点的C末端肽，三聚化（Folcon）结构域和8个组氨酸残基.全长HA前体蛋白是566个残基（Genpept： ACS71642 ）. NR-49734具有62,300道尔顿的理论分子量. nr-49734不展示血凝活动. 每个小瓶在50mM Tris-HCl（pH8）中含有100μg至200μg的NR-49734，其中100mM NaCl和15％甘油.

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产品关键词

NR-49734 带有 C 末端组氨酸标签的 H3 血凝素 (HA) 蛋白来自流感病毒

A/Perth/16/2009 (H3N2)

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NR-43783带有N末端组氨酸标签的N2神经氨酸酶(NA)蛋白,来自流感病毒,A/turkey/Wisconsin/1/1966(H9N2),来自杆状病毒(蛋白质）的重组

NR-43783_ N2 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/turkey/Wisconsin/1/1966 (H9N2), Recombinant from Baculovirus(Proteins)

阅读更多关于 NR-43783带有N末端组氨酸标签的N2神经氨酸酶(NA)蛋白,来自流感病毒,A/turkey/Wisconsin/1/1966(H9N2),来自杆状病毒(蛋白质）的重组

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产品英文名称

产品别名

NR-43783_ N2 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/turkey/Wisconsin/1/1966 (H9N2), Recombinant from Baculovirus(Proteins)
NR-43783 带有 N 末端组氨酸标签的 N2 神经氨酸酶 (NA) 蛋白，来自流感病毒，A/turkey/Wisconsin/1/1966 (H9N2)，来自杆状病毒（蛋白质）的重组

货号/SKU

NR-43783

货号/规格

库存与交货期

3-8周

人民币价格

14000

人民币价格说明

本商品人民币2024年销售价格正在调整中，请等待更新完毕。

本商品的美元价、市场价、零售价、厂商指导价或该商品的曾经展示过的销售价等，并非商品原价，仅供参考。

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A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认

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BEI Resources(ATCC)

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产品基础信息

生物安全等级建议分类：美国、1

产品描述信息

NR-43783?? N2 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/turkey/Wisconsin/1/1966 (H9N2), Recombinant from Baculovirus(Proteins)|Influenza A virus|N2 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/turkey/Wisconsin/1/1966 (H9N2), Recombinant from Baculovirus|-20°C or colder|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: N2 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/turkey/Wisconsin/1/1966 (H9N2), Recombinant from Baculovirus, NR-43783."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.A recombinant form of the N2 neuraminidase (NA) protein from influenza A virus, A/turkey/Wisconsin/1/1966 (H9N2) containing an N-terminal histidine tag was produced in Sf9 insect cells using a baculovirus expression vector system and purified by nickel affinity chromatography. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal eight-histidine tag followed by a 43 amino acid tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site, as described for the 1918 pandemic virus. The full-length NA precursor protein is 469 residues (GenPept: CAE00569). Each vial contains approximately 200 ?g of purified recombinant NA protein in PBS (pH 7.4).NR-43783 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2'-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (4-MUNANA).

主要内容

此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID进行批准. 来自流感病毒的N2神经氨酸酶（NA）蛋白的重组形式，A / Turkey / Wisconsin / 1/1966（含有N-末端组氨酸标签的H9N2）使用杆状病毒表达载体系统在SF9昆虫细胞中产生并通过镍亲和层析纯化.将NA基因的预测的外胚层编码区域融合到编码N-末端8-组氨酸标签的合成基因区段，然后由血管扩张剂刺激的磷蛋白（VASP）和凝血酶切割位点为43个氨基酸四聚化结构域，如上所述1918年的大流行病病毒.全长Na前体蛋白是469个残基（Genpept：Cae00569）. 每个小瓶在PBS中含有大约200μg纯化的重组Na蛋白（pH7.4）. NR-43783基于其切割荧光底物2'的能力，证明NR-43783在功能上活跃 - （4-甲基伏丝）-α-DN-乙酰尿氨酸（4-MUNANA）.

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品牌标识

产品关键词

NR-43783 带有 N 末端组氨酸标签的 N2 神经氨酸酶 (NA) 蛋白

来自流感病毒

A/turkey/Wisconsin/1/1966 (H9N2)

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NR-42002带有N末端组氨酸标签的N1神经氨酸酶(NA)蛋白来自流感病毒,A/PuertoRico/8/1934(H1N1),来自杆状病毒(蛋白质）的重组

NR-42002_ N1 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Puerto Rico/8/1934 (H1N1), Recombinant from Baculovirus(Proteins)

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产品英文名称

产品别名

NR-42002_ N1 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Puerto Rico/8/1934 (H1N1), Recombinant from Baculovirus(Proteins)
NR-42002 带有 N 末端组氨酸标签的 N1 神经氨酸酶 (NA) 蛋白来自流感病毒，A/Puerto Rico/8/1934 (H1N1)，来自杆状病毒（蛋白质）的重组

货号/SKU

NR-42002

货号/规格

库存与交货期

3-8周

人民币价格

14000

人民币价格说明

本商品人民币2024年销售价格正在调整中，请等待更新完毕。

本商品的美元价、市场价、零售价、厂商指导价或该商品的曾经展示过的销售价等，并非商品原价，仅供参考。

试剂海关审批

A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认

国外采购

支持/部分限制一年内购买数量

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BEI Resources(ATCC)

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产品基础信息

生物安全等级建议分类：美国、1

产品描述信息

NR-42002?? N1 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Puerto Rico/8/1934 (H1N1), Recombinant from Baculovirus(Proteins)|Influenza A virus|N1 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Puerto Rico/8/1934 (H1N1), Recombinant from Baculovirus|-20°C|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: N1 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Puerto Rico/8/1934 (H1N1), Recombinant from Baculovirus, NR-42002."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.A recombinant form of the N1 neuraminidase (NA) protein from influenza A virus, A/Puerto Rico/8/1934 (H1N1) containing an N-terminal histidine tag was produced in insect cells using a baculovirus expression vector system. Lot No. 61759226, which is no longer available, was produced in High Five? insect cells and enriched from culture supernatants by nickel affinity chromatography under non-denaturing conditions. Lot No. 63195703 was produced in Spodoptera frugiperda Sf9 cells and purified by nickel affinity chromatography under non-denaturing conditions. The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal eight-histidine tag followed by a 43 amino acid tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site, as described for the 1918 pandemic virus. The predicted protein sequence is shown in Table 1 of the Product Information Sheet. The full-length NA precursor protein is 454 residues (GenPept: ABD77678).NR-42002 was expressed from the same recombinant baculovirus vector as NR-19235, which was purified from cell lysates under denaturing conditions and has not been tested for enzymatic activity. Each vial contains approximately 1 to 5 ?g of partially purified recombinant NA protein in 25 mM phosphate buffer (pH 8.0) with 250 mM NaCl, 250 mM imidazole, and 50% glycerol.NR-42002 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2'-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (4-MUNANA).

主要内容

此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID以进行批准. 来自流感病毒的N1神经氨酸酶（NA）蛋白的重组形式，A / Puerto Rico / 8/1934（H1N1使用杆状病毒表达载体系统在昆虫细胞中产生含有N-末端组氨酸标签.在高五?昆虫细胞中生产不再可用的批号61759226，并在非变性条件下通过镍亲和色谱法富集培养上清液.批号63195703在 Spodoptera frugiperda-/ i> SF9细胞中制备，并通过非变性条件下通过镍亲和层析纯化. 将Na基因的预测的外胚瘤编码区融合到编码N-末端8-组氨酸标签的合成基因区段，然后由血管扩张剂刺激的磷蛋白（VASP）和凝血酶的43个氨基酸四聚化结构域裂解遗址，如1918年大流行病毒所述.预测的蛋白质序列如产品信息表的表1所示.全长Na前体蛋白是454个残基（Genpept：ABD77678）. NR-42002以与NR-19235相同的重组杆状病毒载体表示，其在变性条件下从细胞裂解物中纯化，并且没有已被测试以酶活性. 每个小瓶在25mM磷酸盐缓冲液（pH8.0）中含有约1至5μg的部分纯化的重组Na蛋白，250mM NaCl，250mM咪唑和50％甘油. 基于其切割荧光底物2' - （4-甲基蛋糖）-α-DN-乙酰氨酰氨基丙氨酸（4-MUNANA）的能力，证明-42002在功能上活性活性.

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产品关键词

NR-42002 带有 N 末端组氨酸标签的 N1 神经氨酸酶 (NA) 蛋白来自流感病毒

A/Puerto Rico/8/1934 (H1N1)

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NR-19235N1神经氨酸酶(NA)蛋白,N-末端组氨酸标签来自流感病毒,A/PuertoRico/8/1934(H1N1),来自杆状病毒(蛋白质）的重组

NR-19235_ N1 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Puerto Rico/8/1934 (H1N1), Recombinant from Baculovirus(Proteins)

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产品英文名称

产品别名

NR-19235_ N1 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Puerto Rico/8/1934 (H1N1), Recombinant from Baculovirus(Proteins)
NR-19235 N1 神经氨酸酶 (NA) 蛋白，N-末端组氨酸标签来自流感病毒，A/Puerto Rico/8/1934 (H1N1)，来自杆状病毒（蛋白质）的重组

货号/SKU

NR-19235

货号/规格

库存与交货期

3-8周

人民币价格

14000

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本商品人民币2024年销售价格正在调整中，请等待更新完毕。

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支持/部分限制一年内购买数量

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BEI Resources(ATCC)

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