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产品图片
货号/SKU
NR-52308
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-52308?? Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from Baculovirus(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from Baculovirus| -60°C or colder|F Krammer, F Amanat, S StrohmeierAcknowledgment for publications should read “The following reagent was produced under HHSN272201400008C and obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine Tag, Recombinant from Baculovirus, NR-52308.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the spike (S) glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QJE37812) was produced by transfection of purified plasmid in SF9 insect cells using a baculovirus expression system and purified by nickel affinity chromatography. NR-52308 lacks the signal sequence and contains 1196 residues (ectodomain) of the SARS-CoV-2 spike glycoprotein; the recombinant protein was modified to remove the polybasic S1/S2 cleavage site (RRAR to A; residues 682 to 685), stabilized with a pair of mutations (K986P and V987P, wild type numbering; GenPept: QHD43416) and includes a thrombin cleavage site, T4 foldon trimerization domain and C-terminal hexa-histidine tag. NR-52308 has a theoretical molecular weight of 137,600 daltons. The crystal structure for trimeric S glycoprotein from SARS-CoV-2 has been solved at 3.20 angstrom resolution (PDB: 6VYB).
Note: For a detailed protocol and list of related items, see https://labs.icahn.mssm.edu/krammerlab/covid-19/
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor, allows fusion of the viral and cellular membranes. The S protein is a target for neutralizing antibodies.
NR-52308 reacts with monoclonal anti-histidine tag in western blot analysis. NR-52308 is intended for western blot, ELISA and animal vaccination.
Each vial of NR-52308 contains purified recombinant protein in phosphate buffered saline (PBS).
Note: The long-term stability of this preparation is not known. It is recommended that users confirm the activity of the product if not used within three months of receipt.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
A recombinant form of the spike (S) glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QJE37812) was produced by transfection of purified plasmid in SF9 insect cells using a baculovirus expression system and purified by nickel affinity chromatography. NR-52308 lacks the signal sequence and contains 1196 residues (ectodomain) of the SARS-CoV-2 spike glycoprotein; the recombinant protein was modified to remove the polybasic S1/S2 cleavage site (RRAR to A; residues 682 to 685), stabilized with a pair of mutations (K986P and V987P, wild type numbering; GenPept: QHD43416) and includes a thrombin cleavage site, T4 foldon trimerization domain and C-terminal hexa-histidine tag. NR-52308 has a theoretical molecular weight of 137,600 daltons. The crystal structure for trimeric S glycoprotein from SARS-CoV-2 has been solved at 3.20 angstrom resolution (PDB: 6VYB).
Note: For a detailed protocol and list of related items, see https://labs.icahn.mssm.edu/krammerlab/covid-19/
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor, allows fusion of the viral and cellular membranes. The S protein is a target for neutralizing antibodies.
NR-52308 reacts with monoclonal anti-histidine tag in western blot analysis. NR-52308 is intended for western blot, ELISA and animal vaccination.
Each vial of NR-52308 contains purified recombinant protein in phosphate buffered saline (PBS).
Note: The long-term stability of this preparation is not known. It is recommended that users confirm the activity of the product if not used within three months of receipt.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2），武汉-U-1（Genpept： qhd43416 ）并且包括凝血酶切割位点，T4 folcon三聚化结构域和c末端六核分裂标签. NR-52308具有137,600道尔顿的理论分子量.来自SARS-COV-2的三聚体S糖蛋白的晶体结构已在3.20埃左右求解
分辨率（PDB： 6vyb ）.
注意：有关相关项的详细协议和列表，请参阅https://labs.icahn.mssm.edu/krammerlab/covid-19/
S糖蛋白介导与宿主血管紧张素转化酶2（ACE2）的病毒结合.该蛋白质形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜. S蛋白是中和抗体的靶标.
NR-52308与蛋白质印迹分析中的单克隆抗组氨酸标签反应. NR-52308适用于Western Blot，ELISA和动物疫苗接种.
每个小瓶NR-52308含有磷酸盐缓冲盐水（PBS）的纯化的重组蛋白.
注意：该准备的长期稳定性是未知的.建议用户如果不在收到的三个月内使用，请确认产品的活动.
vipr （病毒病原体资源）提供了附加信息和工具.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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