NR-42002带有N末端组氨酸标签的N1神经氨酸酶(NA)蛋白来自流感病毒,A/PuertoRico/8/1934(H1N1),来自杆状病毒(蛋白质）的重组
产品图片
货号/SKU
NR-42002
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-42002?? N1 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Puerto Rico/8/1934 (H1N1), Recombinant from Baculovirus(Proteins)|Influenza A virus|N1 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Puerto Rico/8/1934 (H1N1), Recombinant from Baculovirus|-20°C|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: N1 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Puerto Rico/8/1934 (H1N1), Recombinant from Baculovirus, NR-42002."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the N1 neuraminidase (NA) protein from influenza A virus, A/Puerto Rico/8/1934 (H1N1) containing an N-terminal histidine tag was produced in insect cells using a baculovirus expression vector system. Lot No. 61759226, which is no longer available, was produced in High Five? insect cells and enriched from culture supernatants by nickel affinity chromatography under non-denaturing conditions. Lot No. 63195703 was produced in Spodoptera frugiperda Sf9 cells and purified by nickel affinity chromatography under non-denaturing conditions.
The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal eight-histidine tag followed by a 43 amino acid tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site, as described for the 1918 pandemic virus. The predicted protein sequence is shown in Table 1 of the Product Information Sheet. The full-length NA precursor protein is 454 residues (GenPept: ABD77678).
NR-42002 was expressed from the same recombinant baculovirus vector as NR-19235, which was purified from cell lysates under denaturing conditions and has not been tested for enzymatic activity.
Each vial contains approximately 1 to 5 ?g of partially purified recombinant NA protein in 25 mM phosphate buffer (pH 8.0) with 250 mM NaCl, 250 mM imidazole, and 50% glycerol.
NR-42002 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2'-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (4-MUNANA).
A recombinant form of the N1 neuraminidase (NA) protein from influenza A virus, A/Puerto Rico/8/1934 (H1N1) containing an N-terminal histidine tag was produced in insect cells using a baculovirus expression vector system. Lot No. 61759226, which is no longer available, was produced in High Five? insect cells and enriched from culture supernatants by nickel affinity chromatography under non-denaturing conditions. Lot No. 63195703 was produced in Spodoptera frugiperda Sf9 cells and purified by nickel affinity chromatography under non-denaturing conditions.
The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal eight-histidine tag followed by a 43 amino acid tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site, as described for the 1918 pandemic virus. The predicted protein sequence is shown in Table 1 of the Product Information Sheet. The full-length NA precursor protein is 454 residues (GenPept: ABD77678).
NR-42002 was expressed from the same recombinant baculovirus vector as NR-19235, which was purified from cell lysates under denaturing conditions and has not been tested for enzymatic activity.
Each vial contains approximately 1 to 5 ?g of partially purified recombinant NA protein in 25 mM phosphate buffer (pH 8.0) with 250 mM NaCl, 250 mM imidazole, and 50% glycerol.
NR-42002 was demonstrated to be functionally active based on its ability to cleave the fluorogenic substrate 2'-(4-methylumbelliferyl)-α-D-N-acetylneuraminic acid (4-MUNANA).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID以进行批准. 来自流感病毒的N1神经氨酸酶（NA）蛋白的重组形式，A / Puerto Rico / 8/1934（H1N1使用杆状病毒表达载体系统在昆虫细胞中产生含有N-末端组氨酸标签.在高五?昆虫细胞中生产不再可用的批号61759226，并在非变性条件下通过镍亲和色谱法富集培养上清液.批号63195703在 Spodoptera frugiperda-/ i> SF9细胞中制备，并通过非变性条件下通过镍亲和层析纯化. 将Na基因的预测的外胚瘤编码区融合到编码N-末端8-组氨酸标签的合成基因区段，然后由血管扩张剂刺激的磷蛋白（VASP）和凝血酶的43个氨基酸四聚化结构域裂解遗址，如1918年大流行病毒所述.预测的蛋白质序列如产品信息表的表1所示.全长Na前体蛋白是454个残基（Genpept：ABD77678）. NR-42002以与NR-19235相同的重组杆状病毒载体表示，其在变性条件下从细胞裂解物中纯化，并且没有已被测试以酶活性. 每个小瓶在25mM磷酸盐缓冲液（pH8.0）中含有约1至5μg的部分纯化的重组Na蛋白，250mM NaCl，250mM咪唑和50％甘油. 基于其切割荧光底物2' - （4-甲基蛋糖）-α-DN-乙酰氨酰氨基丙氨酸（4-MUNANA）的能力，证明-42002在功能上活性活性.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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