NR-19235N1神经氨酸酶(NA)蛋白,N-末端组氨酸标签来自流感病毒,A/PuertoRico/8/1934(H1N1),来自杆状病毒(蛋白质）的重组
产品图片
货号/SKU
NR-19235
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-19235?? N1 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Puerto Rico/8/1934 (H1N1), Recombinant from Baculovirus(Proteins)|Influenza A virus|N1 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Puerto Rico/8/1934 (H1N1), Recombinant from Baculovirus|2°C to 8°C|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: N1 Neuraminidase (NA) Protein with N-Terminal Histidine Tag from Influenza Virus, A/Puerto Rico/8/1934 (H1N1), Recombinant from Baculovirus, NR-19235."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval.
This item is currently in our production queue. Please allow ample time for distribution lots to be made available.
A recombinant form of the N1 neuraminidase (NA) protein from influenza A virus, A/Puerto Rico/8/1934 (H1N1) containing an N-terminal histidine tag was produced in High Five? insect cells using a baculovirus expression vector system and was purified by nickel affinity chromatography under denaturing conditions. The purified protein was refolded by dialysis and filtered.
NR-19235 was expressed from the same recombinant baculovirus vector as NR-42002, which was partially purified from culture supernatants under non-denaturing conditions and is enzymatically active.
The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal eight-histidine tag followed by a 43 amino acid tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site.
Each vial contains approximately 50 to 150 ?g of purified recombinant NA protein in 50 mM Tris-HCl (pH 8.5), 240 mM NaCl, 10 mM KCl, 1 mM EDTA, 0.5 M Arginine, 0.5% Triton X-100, and 1 mM DTT.
NR-19235 has not been tested for enzymatic activity. Previous work at BEI Resources indicated that other influenza virus neuraminidases purified under denaturing conditions and refolded by dialysis are not able to cleave the fluorogenic substrate 2?-(4-methylumbelliferyl)-a-D-N-acetylneuraminic acid (4-MUNANA).
This item is currently in our production queue. Please allow ample time for distribution lots to be made available.
A recombinant form of the N1 neuraminidase (NA) protein from influenza A virus, A/Puerto Rico/8/1934 (H1N1) containing an N-terminal histidine tag was produced in High Five? insect cells using a baculovirus expression vector system and was purified by nickel affinity chromatography under denaturing conditions. The purified protein was refolded by dialysis and filtered.
NR-19235 was expressed from the same recombinant baculovirus vector as NR-42002, which was partially purified from culture supernatants under non-denaturing conditions and is enzymatically active.
The predicted ectodomain coding region of the NA gene was fused to a synthetic gene segment encoding an N-terminal eight-histidine tag followed by a 43 amino acid tetramerization domain from vasodilator-stimulated phosphoprotein (VASP) and a thrombin cleavage site.
Each vial contains approximately 50 to 150 ?g of purified recombinant NA protein in 50 mM Tris-HCl (pH 8.5), 240 mM NaCl, 10 mM KCl, 1 mM EDTA, 0.5 M Arginine, 0.5% Triton X-100, and 1 mM DTT.
NR-19235 has not been tested for enzymatic activity. Previous work at BEI Resources indicated that other influenza virus neuraminidases purified under denaturing conditions and refolded by dialysis are not able to cleave the fluorogenic substrate 2?-(4-methylumbelliferyl)-a-D-N-acetylneuraminic acid (4-MUNANA).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将被发送到北欧以获得批准.
此项目目前处于我们的生产队列中.请允许充足的时间进行分销批次.
使用杆状病毒表达载体系统在高五?昆虫细胞中产生来自流感的N1神经氨氨酸酶（Na）蛋白的N1神经氨氨氨酰蛋白（Na）蛋白的重组形式，含有N-末端组氨酸标签，并使用Baculovirus表达载体系统.在变性条件下通过镍亲和层析纯化.纯化的蛋白质通过透析折叠并过滤.
NR-19235与NR-42002相同的重组杆状病毒载体表示，其在非变性条件下从培养上清液中部分纯化，并酶活性.
将Na基因的预测的外胚瘤编码区域融合到编码N-末端8-组氨酸标签的合成基因区段，然后由血管扩张剂刺激的磷蛋白（VASP）和凝血酶切割位点为43个氨基酸四聚化结构域.
每个小瓶在50mM Tris-HCl（pH8.5），240mM NaCl，10mM KCl，1mM EDTA，0.5M精氨酸，0.5％Triton X-100和1mm中，含有约50至150μg纯化的重组Na蛋白纯化的重组Na蛋白. dtt.
NR-19235尚未测试酶活性.以前的Bei资源的工作表明，在变性条件下纯化的其他流感病毒神经氨酸酶并被透析折叠的透析不能切割荧光底物2'-（4-甲基晶烯烃）-A-D-N-乙酰尿酰胺酸（4-仙甲基）.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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