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Purchase IgE direct from the manufacturer.
MW: 190,000
Extinction Coefficient: 1.46
Frozen in 15 mM potassium phosphate, pH 7.4, with 150 mM NaCl and 0.05% NaN3.
IgE is the least abundant immunoglobulin in plasma, found at a concentration of less that 0.6 micrograms/ml of normal plasma. Elevated IgE levels are found in patients experiencing severe allergic reactions and parasitic infections. In a myeloma condition, IgE is produced by a single clone of plasma cells. The structure of myeloma IgE, however, is normal, and the immunoglobulin purified from a myeloma source is a useful protein for studying immunoglobulin behavior.
Storage:
Purity: >=95% by SDS-PAGE
Prepared from human myeloma plasma, Kappa shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.
Lab products are laboratory reagents and are not to be administered to humans or used for any drug purpose. For research or further manufacturing use only.
Product Citations:
Pérez Ruiz, Elena, Marijn Kemper, Dragana Spasic, Ann Gils, Leo J. van IJzendoorn, Jeroen Lammertyn, and Menno WJ Prins. "Probing the Force-induced Dissociation of Aptamer-Protein Complexes."?Analytical chemistry(2014).
Pérez-Ruiz E, Lammertyn J, Spasic D. Evaluation of different strategies for magnetic particle functionalization with DNA aptamers. N Biotechnol.2016 Dec 25;33(6):755-762. doi: 10.1016/j.nbt.2016.06.1459. Epub 2016 Jun 16.
Arghir J, et al. Improved surface plasmon resonance biosensing using silanized optical fibers. Sensors and Actuators B: Chemical, volume 216 September 2015 pg 518-526.
Ref:
Ishiaka, K. 1985. Methods Enzymol. 116, 76.
