NR-53528_PstS1(基因Rv0934,非酰化）,来自结核分枝杆菌的纯化天然蛋白质,菌株H37Rv(蛋白质）
产品图片
货号/SKU
NR-53528
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-53528?? PstS1 (Gene Rv0934, Non-Acylated), Purified Native Protein from Mycobacterium tuberculosis, Strain H37Rv(Proteins)|Mycobacterium tuberculosis|PstS1 (Gene Rv0934, Non-Acylated), Purified Native Protein from Mycobacterium tuberculosis, Strain H37Rv|-80°C or colder|BEI Resources or NIH - TB Vaccine Testing and Research Materials ContractAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: PstS1 (Gene Rv0934, Non-Acylated), Purified Native Protein from Mycobacterium tuberculosis, Strain H37Rv, NR-53528."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-53528 is a preparation of periplasmic phosphate-binding lipoprotein, PstS1 (Gene Rv0934, non-acylated; also referred to as PhoS1), purified from the culture filtrate of Mycobacterium tuberculosis, strain H37Rv. The proteins soluble in 40% ammonium sulfate were precipitated by 70% saturated ammonium sulfate solution, suspended in ConA binding buffer and applied to a ConA column. ConA elution buffer, containing alpha-D-mannopyranoside, was used to elute the bound proteins. The bound material was applied to a phenyl sepharose column and eluted using a gradient of decreasing ammonium sulfate. Fractions containing pure 38kDa were pooled and dialyzed.
Note: The cell wall-associated and culture filtrate forms of PhoS1 seem to differ in their hydrophobic characteristics. However, it has not been determined if there is a chemical difference between the cell wall and culture filtrate forms of the PhoS1 homologue.
Each vial contains approximately 100 ?g of lyophilized, purified NR-53528 in 10 mM ammonium bicarbonate.
Note: PstS1 is soluble in 100 mM to 500 mM aqueous buffered salt solutions, such as phosphate buffered saline. A 10 mM ammonium bicarbonate solution can also be used.
NR-53528 is a preparation of periplasmic phosphate-binding lipoprotein, PstS1 (Gene Rv0934, non-acylated; also referred to as PhoS1), purified from the culture filtrate of Mycobacterium tuberculosis, strain H37Rv. The proteins soluble in 40% ammonium sulfate were precipitated by 70% saturated ammonium sulfate solution, suspended in ConA binding buffer and applied to a ConA column. ConA elution buffer, containing alpha-D-mannopyranoside, was used to elute the bound proteins. The bound material was applied to a phenyl sepharose column and eluted using a gradient of decreasing ammonium sulfate. Fractions containing pure 38kDa were pooled and dialyzed.
Note: The cell wall-associated and culture filtrate forms of PhoS1 seem to differ in their hydrophobic characteristics. However, it has not been determined if there is a chemical difference between the cell wall and culture filtrate forms of the PhoS1 homologue.
Each vial contains approximately 100 ?g of lyophilized, purified NR-53528 in 10 mM ammonium bicarbonate.
Note: PstS1 is soluble in 100 mM to 500 mM aqueous buffered salt solutions, such as phosphate buffered saline. A 10 mM ammonium bicarbonate solution can also be used.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
NR-53528是磷酸盐结合脂蛋白的制备，PSTS1（基因RV0934，非酰化;也称为PHOS1），从培养滤液中纯化，菌分H37RV菌株H37RV.溶于40％硫酸铵的蛋白质被70％饱和硫酸铵溶液沉淀，悬浮在Cona结合缓冲液中并施加到Cona柱上. Cona洗脱缓冲液含有α-D-甘露糖苷，用于洗脱结合的蛋白质.将染色的材料施加到苯琼糖柱上并用减少硫酸铵的梯度洗脱.合并含有纯38KDA的级分和透析.
注意：细胞壁相关和培养滤液形式的PHOS1似乎有所不同
他们的疏水特性.然而，如果细胞壁和培养滤液形式之间存在化学差异，则尚未确定pHOS1同源物之间的化学差异.
每个小瓶在10mM碳酸氢铵中含有约100μg的冻干，纯化的NR-53528.
注意：PSTS1可溶于100mm至500mM水缓冲盐溶液，例如磷酸盐缓冲盐水.也可以使用10mM碳酸氢铵溶液.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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