产品图片
货号/SKU
NR-53525
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-53525?? Ag85A (Gene Rv3804c), Purified Native Protein from Mycobacterium tuberculosis, Strain H37Rv(Proteins)|Mycobacterium tuberculosis|Ag85A (Gene Rv3804c), Purified Native Protein from Mycobacterium tuberculosis, Strain H37Rv|-80°C or colder|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Ag85A (Gene Rv3804c), Purified Native Protein from Mycobacterium tuberculosis, Strain H37Rv, NR-53525."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-53525 is a preparation of antigen 85A (Ag85A) protein purified from the culture filtrate proteins of Mycobacterium tuberculosis, strain H37Rv.
The culture filtrate proteins were precipitated from culture filtrate with 40% ammonium sulfate. The precipitate was suspended and applied to phenyl sepharose HPLC. Antigen 85 was obtained by increasing the pH and eluting with a high concentration of ethylene glycol. Fractionation of the Ag85 complex was performed by size exclusion chromatography to yield the Ag85A component. The purified product was dialyzed in 10 mM ammonium bicarbonate.
Each vial contains approximately 100 ?g of lyophilized, purified Ag85A from Mycobacterium tuberculosis, strain H37Rv in 10 mM ammonium bicarbonate.
Note: NR-53525 is soluble in 100 mM to 500 mM aqueous buffered salt solutions, such as phosphate buffered saline. A 10 mM ammonium bicarbonate solution can also be used.
NR-53525 is a preparation of antigen 85A (Ag85A) protein purified from the culture filtrate proteins of Mycobacterium tuberculosis, strain H37Rv.
The culture filtrate proteins were precipitated from culture filtrate with 40% ammonium sulfate. The precipitate was suspended and applied to phenyl sepharose HPLC. Antigen 85 was obtained by increasing the pH and eluting with a high concentration of ethylene glycol. Fractionation of the Ag85 complex was performed by size exclusion chromatography to yield the Ag85A component. The purified product was dialyzed in 10 mM ammonium bicarbonate.
Each vial contains approximately 100 ?g of lyophilized, purified Ag85A from Mycobacterium tuberculosis, strain H37Rv in 10 mM ammonium bicarbonate.
Note: NR-53525 is soluble in 100 mM to 500 mM aqueous buffered salt solutions, such as phosphate buffered saline. A 10 mM ammonium bicarbonate solution can also be used.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
NR-53525是一种制备抗原85a(Ag85a)蛋白的蛋白质从培养滤液蛋白质的培养物蛋白蛋白,菌株H37RV菌株.
将培养滤液蛋白质从培养滤液中沉淀,硫酸40%硫酸铵.将沉淀物悬浮并施加到苯基琼脂糖HPLC上.通过增加pH并用高浓度的乙二醇洗脱来获得抗原85.通过尺寸排阻色谱法进行AG85复合物的分馏,得到Ag85a组分.将纯化的产物在10毫米中透析
碳酸氢铵.
每个小瓶含有大约100μg的冻干,纯化的Ag85a,从
注意:NR-53525可溶于100mm至500mM水缓冲盐溶液,例如磷酸盐缓冲盐水.也可以使用10mM碳酸氢铵溶液.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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