NR-51315土拉弗朗西斯菌亚种.novicida,“双等位基因”转座子突变库,板33(突变细菌）
产品图片
货号/SKU
NR-51315
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、2
产品描述信息
NR-51315??Francisella tularensis subsp. novicida, "Two-Allele" Transposon Mutant Library, Plate 33(Mutant Bacteria)|Francisella tularensis subsp. novicida|"Two-Allele" Transposon Mutant Library, Plate 33|-80°C or colder|C ManoilAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Francisella tularensis subsp. novicida, "Two-Allele" Transposon Mutant Library, Plate 33, NR-51315."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources does not confirm or validate individual mutants provided by the contributor.
A comprehensive 16,508-member transposon mutant library of sequence-defined transposon insertion mutants of Francisella tularensis subsp. novicida, strain U112 was prepared to allow the systematic identification of virulence determinants and other factors associated with Francisella pathogenesis. Genes refractory to insertional inactivation helped define the genes essential for viability of the organism.
To facilitate genome-scale screening using the mutant collection, a “two-allele” single-colony purified sublibrary, made up of approximately two purified mutants per gene, was assembled.
NR-51315 contains 18 mutants that were not available at the time of the release of Plates 1 to 32 of the “two-allele” 3,050-member sublibrary.
Francisella tularensis subsp. novicida, strain U112 is excluded from Select Agent status. Please see CDC Select Agent Program, Notification of Exclusion.
Each inoculated well of the 96-well plate contains approximately 50 ?L of culture in Tryptic Soy broth containing 0.1% L-cysteine and 10 ?g/mL kanamycin supplemented with 5% glycerol.
Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources does not confirm or validate individual mutants provided by the contributor.
A comprehensive 16,508-member transposon mutant library of sequence-defined transposon insertion mutants of Francisella tularensis subsp. novicida, strain U112 was prepared to allow the systematic identification of virulence determinants and other factors associated with Francisella pathogenesis. Genes refractory to insertional inactivation helped define the genes essential for viability of the organism.
To facilitate genome-scale screening using the mutant collection, a “two-allele” single-colony purified sublibrary, made up of approximately two purified mutants per gene, was assembled.
NR-51315 contains 18 mutants that were not available at the time of the release of Plates 1 to 32 of the “two-allele” 3,050-member sublibrary.
Francisella tularensis subsp. novicida, strain U112 is excluded from Select Agent status. Please see CDC Select Agent Program, Notification of Exclusion.
Each inoculated well of the 96-well plate contains approximately 50 ?L of culture in Tryptic Soy broth containing 0.1% L-cysteine and 10 ?g/mL kanamycin supplemented with 5% glycerol.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
96孔格式的生产增加了相邻井之间的交叉污染的风险.应纯化各个克隆（例如，使用良好的微生物实践的单菌落分离和纯化）并在使用前进行序列验证. Bei Resources不确认或验证贡献者提供的个体突变体.
综合的16,508-构成序列定义的转座子插入突变体的序列定义的转座子插入突变体突变体 Francisella tularensiss subsp. Novicida ，菌株U112准备允许系统鉴定毒力决定因素和与毛蕊菌相关的其他因素的发病机制.插入灭活的基因有助于定义生物体活力所必需的基因.
为了促进使用突变收集的基因组抑制，组装了由每种基因大约两种纯化的突变体组成的“双等位基因”单菌落纯化的子纤维结构.
NR-51315含有18个突变体，其在释放板1至32的“双等位基因”3,050-成员子锋中的释放时不可用.＆nbsp;
rancisella tularentensis subsp. novicida ，应变u112从SELECT代理状态中排除.请参阅 ?? CDC选择代理程序，排除通知
96孔板的每个接种井在含有0.1％L-半胱氨酸的胰蛋白酶大豆肉汤中含有大约50μl培养物，并补充有5％甘油的10μg/ ml卡那霉素.
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