NR-51290土拉弗朗西斯菌亚种.novicida,“双等位基因”转座子突变库,板8(tnfn1_pw060323p08)(突变细菌）
产品图片
货号/SKU
NR-51290
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、2
产品描述信息
NR-51290??Francisella tularensis subsp. novicida, "Two-Allele" Transposon Mutant Library, Plate 8 (tnfn1_pw060323p08)(Mutant Bacteria)|Francisella tularensis subsp. novicida|"Two-Allele" Transposon Mutant Library, Plate 8 (tnfn1_pw060323p08)|-80°C or colder|C ManoilAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Francisella tularensis subsp. novicida, "Two-Allele" Transposon Mutant Library, Plate 8 (tnfn1_pw060323p08), NR-51290."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A comprehensive 16,508-member transposon mutant library of sequence-defined transposon insertion mutants of Francisella tularensis subsp. novicida, strain U112 was prepared to allow the systematic identification of virulence determinants and other factors associated with Francisella pathogenesis. Genes refractory to insertional inactivation helped define the genes essential for viability of the organism.
To facilitate genome-scale screening using the mutant collection, a “two-allele” single-colony purified sublibrary, made up of approximately two purified mutants per gene, was assembled.
NR-51290 represents plate 8 (tnfn1_pw060323p08) of the “two-allele” 3,050-member sublibrary. Detailed information for each mutant is shown on the Product Information Sheet.
Francisella tularensis subsp. novicida, strain U112 is excluded from Select Agent status. Please see CDC Select Agent Program, Notification of Exclusion.
Each inoculated well of the 96-well plate contains approximately 50 ?L of culture in Tryptic Soy broth containing 0.1% L-cysteine and 10 ?g/mL kanamycin supplemented with 5% glycerol.
Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources does not confirm or validate individual mutants provided by the contributor.
A comprehensive 16,508-member transposon mutant library of sequence-defined transposon insertion mutants of Francisella tularensis subsp. novicida, strain U112 was prepared to allow the systematic identification of virulence determinants and other factors associated with Francisella pathogenesis. Genes refractory to insertional inactivation helped define the genes essential for viability of the organism.
To facilitate genome-scale screening using the mutant collection, a “two-allele” single-colony purified sublibrary, made up of approximately two purified mutants per gene, was assembled.
NR-51290 represents plate 8 (tnfn1_pw060323p08) of the “two-allele” 3,050-member sublibrary. Detailed information for each mutant is shown on the Product Information Sheet.
Francisella tularensis subsp. novicida, strain U112 is excluded from Select Agent status. Please see CDC Select Agent Program, Notification of Exclusion.
Each inoculated well of the 96-well plate contains approximately 50 ?L of culture in Tryptic Soy broth containing 0.1% L-cysteine and 10 ?g/mL kanamycin supplemented with 5% glycerol.
Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources does not confirm or validate individual mutants provided by the contributor.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送到NIAID进行批准. 一个全面的16,508名成员
序列定义的转座子插入的转座突变库
突变体＆nbsp; francis ella tularensis＆nbsp; subsp. novicida ，strats u112＆nbsp; 是准备允许系统
鉴定毒力决定因素和与弗朗西斯相关的其他因素 ella 发病机制.基因难以插入灭活
帮助定义生物生存能力所必需的基因.促进
使用突变集合的基因组绘制筛选，“双等位基因”
单菌落净化的子纤维结构，由大约两种纯化组成
组装每个基因的突变体.
NR-51290表示板8（TNFN1_PW060323P08）
“双等位基因”3,050-成员子博.＆nbsp;产品信息表上显示了每个突变体的详细信息. 弗朗西亚tularensis subsp. novicida ，菌株U112从SELECT代理状态中排除.请参阅 CDC选择代理程序，排除通知.
96孔板在胰蛋白酶汤中含有大约50μl培养物
含有0.1％的L-半胱氨酸和10μg/ ml卡那霉素，其补充有5％甘油.＆nbsp; 生产
在96孔格式的情况下，增加了交叉污染的风险
相邻的井.应纯化单个克隆（例如单殖民地
使用良好的微生物实践分离和纯化）和
使用前序列验证. Bei资源不确认或验证
贡献者提供的个体突变体.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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