产品图片
货号/SKU
NR-52723
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-52723??Mycobacterium tuberculosis, H37Rv, Extracellular Vesicle-Depleted Culture Filtrate Proteins(Antigen Preparations)|Mycobacterium tuberculosis|H37Rv, Extracellular Vesicle-Depleted Culture Filtrate Proteins| 4°C
or colder|K DobosAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain H37Rv, Extracellular Vesicle-Depleted Culture Filtrate Proteins, NR-52723."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-52723 is a preparation of extracellular vesicle (EV)-depleted culture filtrate proteins (CFP) from Mycobacterium tuberculosis (M. tuberculosis), strain H37Rv. Mycobacterial EVs are involved in disease pathogenesis through host-pathogen interactions, including modulation of immune response and virulence, and may have potential as disease biomarkers and in vaccine and therapeutic development.
The culture was grown to late log phase in glycerol-alanine-salts medium. The culture supernatant was harvested from the live cells and the resulting CFP passed through a 100 kDa ultrafiltration device to remove extracellular vesicles and other large protein aggregates. Protein was quantitated by bicinchoninic acid (BCA) assay.
Each vial of NR-52723 contains approximately 250 ?g of extracellular vesicle-depleted culture filtrate proteins from M. tuberculosis, strain H37Rv in 10 mM ammonium bicarbonate.
NR-52723 is a preparation of extracellular vesicle (EV)-depleted culture filtrate proteins (CFP) from Mycobacterium tuberculosis (M. tuberculosis), strain H37Rv. Mycobacterial EVs are involved in disease pathogenesis through host-pathogen interactions, including modulation of immune response and virulence, and may have potential as disease biomarkers and in vaccine and therapeutic development.
The culture was grown to late log phase in glycerol-alanine-salts medium. The culture supernatant was harvested from the live cells and the resulting CFP passed through a 100 kDa ultrafiltration device to remove extracellular vesicles and other large protein aggregates. Protein was quantitated by bicinchoninic acid (BCA) assay.
Each vial of NR-52723 contains approximately 250 ?g of extracellular vesicle-depleted culture filtrate proteins from M. tuberculosis, strain H37Rv in 10 mM ammonium bicarbonate.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
NR-52723是一种制备来自结核分枝杆菌(结核病) - 菌株H37RV的细胞外囊泡(EV)培养滤液蛋白(CFP)的制备.通过宿主病原体相互作用,包括调节免疫应答和毒力,并且可能具有疾病生物标志物和疫苗和治疗发育的疾病发病性.
将培养物生长至甘油 - 丙氨酸 - 盐培养基中的晚记物相.从活细胞收获培养上清液,得到的CFP通过100kDa超滤装置以除去细胞外囊泡和其他大蛋白质聚集体.通过双式碳酸(BCA)测定法定量蛋白质.
每个小瓶NR-52723含有大约250μg的细胞外囊泡耗尽培养滤液滤液蛋白.结核病,菌株H37RV在10mM碳酸氢铵中.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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