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产品图片
货号/SKU
NR-13291
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-13291?? Plasmid pMRLB.38 Containing Gene Rv3803c (Protein Mpt51) from Mycobacterium tuberculosis(Plasmid/Vectors)|Mycobacterium tuberculosis|Plasmid pMRLB.38 Containing Gene Rv3803c (Protein Mpt51) from Mycobacterium tuberculosis|-20°C or colder|NIH - TB Vaccine Testing and Research Materials ContractAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pMRLB.38 Containing Gene Rv3803c (Protein Mpt51) from Mycobacterium tuberculosis, NR-13291."|Quantity limit for this item is 2 per year. Orders over this limit will be sent to NIAID for approval before shipment.
NR-13291 is a recombinant expression vector containing Mycobacterium tuberculosis gene Rv3803c, which encodes the secreted antigen protein MPT51 (also referred to as the fibronectin-binding protein). Gene Rv3803c was amplified by PCR and cloned into pET23b for expression in Escherichia coli. The gene was cloned without a signal sequence. The expressed protein is histidine-tagged and has an observed molecular weight of 31 kDa. The expected purified protein yield from a one liter culture is approximately 4 mg.
Note: Plasmid pMRLB.38 contains the gene required for ampicillin (Ap) resistance. The recommended concentration of Ap in culture is 130 ?g/mL.
Each vial contains approximately 1 ?g of plasmid DNA in 10 mM Tris-HCl, pH 8.5. Lot 04.EC.12.07 contains approximately 1 ?g of plasmid DNA in 10 mM Tris-HCl, pH 7.5.
NR-13291 is a recombinant expression vector containing Mycobacterium tuberculosis gene Rv3803c, which encodes the secreted antigen protein MPT51 (also referred to as the fibronectin-binding protein). Gene Rv3803c was amplified by PCR and cloned into pET23b for expression in Escherichia coli. The gene was cloned without a signal sequence. The expressed protein is histidine-tagged and has an observed molecular weight of 31 kDa. The expected purified protein yield from a one liter culture is approximately 4 mg.
Note: Plasmid pMRLB.38 contains the gene required for ampicillin (Ap) resistance. The recommended concentration of Ap in culture is 130 ?g/mL.
Each vial contains approximately 1 ?g of plasmid DNA in 10 mM Tris-HCl, pH 8.5. Lot 04.EC.12.07 contains approximately 1 ?g of plasmid DNA in 10 mM Tris-HCl, pH 7.5.
主要内容
此项目的数量限制为每年2.此限制的订单将在发货前发送到NIAID进行批准. NR-13291是一种重组表达载体，其含有分枝杆菌分枝杆菌菌分基因Rv3803c，其编码分泌的抗原.蛋白质MPT51（也称为纤连蛋白结合蛋白）.通过PCR扩增基因RV3803C，并克隆到PET23B中以表达在大肠杆菌中.在没有信号序列的情况下克隆基因.表达的蛋白质是组氨酸标记的并且具有观察到的31kDa的分子量.来自一升培养的预期纯化的蛋白质产量约为4毫克. 质粒pMRLB.38含有氨苄青霉素（AP）抗性所需的基因.推荐的培养物浓度为130μg/ ml. 每个小瓶在10mM Tris-HCl中含有约1μg质粒DNA，pH8.5.批次04.EC.12.07在10mM Tris-HCl，pH 7.5中含有约1μg质粒DNA.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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