产品图片
货号/SKU
NR-52565
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-52565?? Modified pαH Vector Containing the Human Angiotensin-Converting Enzyme 2(Plasmid/Vectors)||Modified pαH Vector Containing the Human Angiotensin-Converting Enzyme 2| -20°C or colder|B GrahamAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Modified pαH Vector Containing the Human Angiotensin-Converting Enzyme 2, NR-52565.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Note: The label on the vial is incorrect; the expressed protein has a Strep-tag? II.
NR-52565 is intended for producing pseudotyped particles/pseudovirions. NR-52565 is not intended for recombinant soluble protein expression.
The vector for the human angiotensin-converting enzyme 2 (ACE2) (GenBank: GQ262784) was designed by subcloning the ACE2 mRNA sequence into the pαH mammalian expression vector, which was modified by subcloning an HRV3C protease cleavage site, and the tags octa-histidine and Strep-tag? II downstream of the open reading frame. NR-52565 contains the beta-lactamase gene, bla, to provide transformant selection through ampicillin resistance in Escherichia coli (E. coli). The complete plasmid sequence and map are provided on the BEI Resources webpage. The plasmid was produced in E. coli and extracted.
ACE2 is a human receptor expressed widely, including in the heart, kidney, small intestine and lung, and is involved in the regulation of hypertension and other cardiovascular diseases. The SARS-Related Coronavirus 2 spike glycoprotein mediates viral binding to the host ACE2 receptor. This protein forms a trimer, and when bound to ACE2, allows fusion of the viral and cellular membranes, allowing viral entry and replication. The interaction of ACE2 and the spike glycoprotein during viral infection is currently under study.
Each vial contains plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0). The vial should be centrifuged prior to opening. Note: The contents of the vial should be used to replicate the plasmid in E. coli prior to mammalian expression.
Note: The label on the vial is incorrect; the expressed protein has a Strep-tag? II.
NR-52565 is intended for producing pseudotyped particles/pseudovirions. NR-52565 is not intended for recombinant soluble protein expression.
The vector for the human angiotensin-converting enzyme 2 (ACE2) (GenBank: GQ262784) was designed by subcloning the ACE2 mRNA sequence into the pαH mammalian expression vector, which was modified by subcloning an HRV3C protease cleavage site, and the tags octa-histidine and Strep-tag? II downstream of the open reading frame. NR-52565 contains the beta-lactamase gene, bla, to provide transformant selection through ampicillin resistance in Escherichia coli (E. coli). The complete plasmid sequence and map are provided on the BEI Resources webpage. The plasmid was produced in E. coli and extracted.
ACE2 is a human receptor expressed widely, including in the heart, kidney, small intestine and lung, and is involved in the regulation of hypertension and other cardiovascular diseases. The SARS-Related Coronavirus 2 spike glycoprotein mediates viral binding to the host ACE2 receptor. This protein forms a trimer, and when bound to ACE2, allows fusion of the viral and cellular membranes, allowing viral entry and replication. The interaction of ACE2 and the spike glycoprotein during viral infection is currently under study.
Each vial contains plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8.0). The vial should be centrifuged prior to opening. Note: The contents of the vial should be used to replicate the plasmid in E. coli prior to mammalian expression.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.通过此限制的订单将在发货前发送至NIAID进行批准.
注意：小瓶上的标签不正确;表达的蛋白质具有 strep -tag ? ii .
NR-52565旨在产生假型颗粒/假致毒液. NR-52565不适用于重组可溶性蛋白表达.
设计了人血管紧张素转换酶2（ACE2）的载体（Genbank： GQ262784 ）是设计的通过将ACE2 mRNA序列亚克隆到Pαh哺乳动物表达载体中，通过亚克隆HRV3C蛋白酶切割位点进行修饰，以及标签Octa-组氨酸和 STREP -TAG ? II在开放阅读框的下游. NR-52565含有β-内酰胺酶基因， BLA ，以通过氨苄青霉素抗性在大肠杆菌（大肠杆菌）中提供转化体选择.在北部资源网页上提供完整的质粒序列和地图.在 e中制备质粒. Coli 并提取.
ACE2是一种广泛表达的人类受体，包括心脏，肾，小肠和肺，并参与调节高血压和其他心血管疾病. SARS相关的冠状病毒2穗糖蛋白介导病毒结合到宿主ACE2受体.该蛋白质形成三聚体，并且当与ACE2结合时，允许融合病毒和细胞膜，允许病毒进入和复制.在病毒感染期间ACE2和穗糖蛋白的相互作用目前正在研究中.
每个小瓶在Te缓冲液中含有质粒DNA（10mM Tris-HCl，1mM EDTA，pH8.0）.在打开之前应该离心小瓶. 注意：Vial的内容应该用于复制 e中的质粒. Coli 在哺乳动物表达之前.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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