NR-49771心脏地带病毒,MO-4(病毒）

NR-49771??Heartland virus, MO-4(Viruses)|Heartland virus|MO-4|-60°C or colder|World Reference Center for Emerging Viruses and ArbovirusesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH, as part of the WRCEVA program: Heartland Virus, MO-4, NR-49771."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.Heartland virus (HRTV), MO-4 was isolated from a human in Missouri, USA, in 2009. The patient reported tick exposure and had ehrlichiosis-like symptoms. Upon co-culture of peripheral blood mononuclear cells with DH82 canine monocytes for detection of Ehrlichia chaffeensis, a novel phlebovirus was isolated instead. Supernatants were transferred to Vero E6 cells for virus propagation.Complete nucleotide sequences of the small (S; GenBank: JX005842, JX005843), medium (M; GenBank: JX005844, JX005845), and large (L; GenBank: JX005846, JX005847) RNA genome segments of HRTV have been determined.Each vial contains approximately 1 mL of cell lysate and supernatant from Cercopithecus aethiops kidney epithelial cells (Vero: ATCC ^? CCL-81?) infected with HRTV, MO-4.Note: If homogeneity is required for your intended use, please purify prior to initiating work.Additional information and tools are available at ViPR (Virus Pathogen Resource).