NR-40277单克隆抗克里米亚-刚果出血热病毒Pre-Gc糖蛋白,克隆11E7(体外生产)(单克隆抗体）

NR-40277?? Monoclonal Anti-Crimean-Congo Hemorrhagic Fever Virus Pre-Gc Glycoprotein, Clone 11E7 (produced in vitro)(Monoclonal Antibodies)|Crimean-Congo hemorrhagic fever virus|Monoclonal Anti-Crimean-Congo Hemorrhagic Fever Virus Pre-Gc Glycoprotein, Clone 11E7 (produced in vitro)|-20°C or colder|CS SchmaljohnAcknowledgment for publications should read "The following reagent was obtained from the Joel M. Dalrymple - Clarence J. Peters USAMRIID Antibody Collection through BEI Resources, NIAID, NIH: Monoclonal Anti-Crimean-Congo Hemorrhagic Fever Virus Pre-Gc Glycoprotein, Clone 11E7 (produced in vitro), NR-40277."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.Antibody Class: IgG2aκMouse monoclonal antibody prepared against the Crimean-Congo hemorrhagic fever virus (CCHFV) Pre-Gc glycoprotein was purified from clone 11E7 hybridoma supernatant by protein G affinity chromatography. The B cell hybridoma was generated by the fusion of Sp2/0-Ag14 mouse myeloma cells with splenocytes from BALB/c mice immunized with protein A sepharose-bound CCHFV glycoprotein-antibody complexes as described by Bertolotti-Ciarlet et al. The epitope recognized by the clone 11E7 antibody is contained within a highly conserved region between amino acids 1443 and 1566 of the M segment of the IbAr10200 strain of CCHFV.This reagent is part of the Joel M. Dalrymple - Clarence J. Peters USAMRIID Antibody Collection.Each vial of NR-40277 contains approximately 100 ?L of purified monoclonal antibody in PBS.Antigen-reactivity of NR-40277 was confirmed in indirect immunofluorescence assays using Vero E6 cells infected with CCHFV, plaque reduction neutralization tests, and/or indirect ELISA. Clone 11E7 antibody is also reported to function in flow cytometry, immunoprecipitation, radioimmunoassay, and western blot applications, to partially protect suckling mice from lethal CCHFV challenge, and to recognize an epitope that is conserved among several CCHFV strains.