NR-52724来自SARS相关冠状病毒2的刺突糖蛋白(稳定）,Wuhan-Hu-1带有C末端组氨酸和Twin-Strep?标签,来自HEK293细胞(蛋白质）的重组
产品图片
货号/SKU
NR-52724
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-52724?? Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine and Twin-Strep? Tags, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine and Twin-Strep? Tags, Recombinant from HEK293 Cells|-60°C or colder|DM MagnaniAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine and Twin-Strep? Tags, Recombinant from HEK293 Cells, NR-52724.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the spike (S) glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QHD43416) was produced by transient transfection of the plasmid VRC7471 into human embryonic kidney HEK293 cells, purified by immobilized metal affinity and StrepTactin? Sepharose? chromatography, and dialyzed into buffer. NR-52724 lacks the signal sequence and contains 1194 residues (ectodomain) of the SARS-CoV-2 S glycoprotein; the recombinant protein was stabilized by substitution at the furin S1/S2 cleavage site (RRAR→GSAS; residues 682 to 685) and KV→PP mutations (residues 986 and 987), and includes a T4 foldon trimerization domain, HRV3C protease cleavage site, and C-terminal octa-histidine and Twin-Strep? (TST) tags. NR-52724 has a theoretical molecular weight of 140,700 daltons. The crystal structure for trimeric S glycoprotein from SARS-CoV-2 has been solved at 3.46 ? resolution (PDB: 6VSB).
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. The S protein is a target for neutralizing antibodies.
Each vial contains approximately 100 ?L of NR-52724 in phosphate buffered saline (PBS; pH 7.0).
Note: The long-term stability of this preparation is not known at this time. It is recommended that users confirm the activity of the product if not used within three months of receipt.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
A recombinant form of the spike (S) glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QHD43416) was produced by transient transfection of the plasmid VRC7471 into human embryonic kidney HEK293 cells, purified by immobilized metal affinity and StrepTactin? Sepharose? chromatography, and dialyzed into buffer. NR-52724 lacks the signal sequence and contains 1194 residues (ectodomain) of the SARS-CoV-2 S glycoprotein; the recombinant protein was stabilized by substitution at the furin S1/S2 cleavage site (RRAR→GSAS; residues 682 to 685) and KV→PP mutations (residues 986 and 987), and includes a T4 foldon trimerization domain, HRV3C protease cleavage site, and C-terminal octa-histidine and Twin-Strep? (TST) tags. NR-52724 has a theoretical molecular weight of 140,700 daltons. The crystal structure for trimeric S glycoprotein from SARS-CoV-2 has been solved at 3.46 ? resolution (PDB: 6VSB).
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. The S protein is a target for neutralizing antibodies.
Each vial contains approximately 100 ?L of NR-52724 in phosphate buffered saline (PBS; pH 7.0).
Note: The long-term stability of this preparation is not known at this time. It is recommended that users confirm the activity of the product if not used within three months of receipt.
Additional information and tools are available at ViPR (Virus Pathogen Resource).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2），武汉-U-1（Genpept：）通过瞬态转染质粒VRC7471进入人胚胎肾HEK293细胞，通过固定化的金属亲和力和StrefactIN TM Sepharose ?色谱法纯化，并透析到缓冲液中. NR-52724缺乏信号序列并含有1194个残基（Ectodomain）的SARS-COV-2S糖蛋白;通过在Furin S1 / S2切割位点（RrAR→GSAs;残基682至685）和KV→PP突变（残基986和987）中的替代物稳定重组蛋白，并且包括T4粘性三聚化结构域，HRV3C蛋白酶切割位点，和C-末端Octa-mostitine和Twin-strep ?＆nbsp;（tst）标签. NR-52724具有140,700道尔顿的理论分子量. SARS-COV-2中三聚糖蛋白的晶体结构已在3.46埃分辨率（PDB： 6vsb ）.
S糖蛋白介导与宿主血管紧张素转化酶2（ACE2）的病毒结合.该蛋白质形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜. S蛋白是中和抗体的靶标.
每个小瓶在磷酸盐缓冲盐水中含有大约100μl的NR-52724（PBS; pH 7.0）.
注意：此时不知道该准备的长期稳定性.建议用户如果不在收到的三个月内使用，请确认产品的活动.
vipr （病毒病原体资源）提供了附加信息和工具.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
除了为传染病界提供材料外，BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势，包括安全存储、社区访问和分发；同时保护存款人的知识产权。只要有需要，BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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