Wuhan-Hu-1 带有 C 末端组氨酸和 Twin-Strep? 标签

NR-53589?? Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine and Twin-Strep? Tags, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine and Twin-Strep? Tags, Recombinant from HEK293 Cells| -20°C or colder|DN Sather, P Myler, J McLellanAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine and Twin-Strep^? Tags, Recombinant from HEK293 Cells, NR-53589.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A recombinant form of the spike (S) glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QHD43416) was produced by transient transfection of a previously published construct (VRC7471) into human embryonic kidney HEK293 cells and purified by immobilized metal affinity and gel filtration chromatography. NR-53589 lacks the native signal sequence and contains 1194 residues (ectodomain) of the SARS-CoV-2 S glycoprotein; the recombinant protein was stabilized by substitution at the furin S1/S2 cleavage site (RRAR→GSAS; residues 682 to 685) and KV→PP mutations (residues 986 and 987), and includes a T4 foldon trimerization domain, HRV3C protease cleavage site, and C-terminal octa-histidine and Twin-Strep^? tags. NR-53589 has a theoretical molecular weight of 140,700 daltons. The crystal structure for trimeric S glycoprotein from SARS-CoV-2 has been solved at 3.46 ? resolution (PDB: 6VSB). The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor, allows fusion of the viral and cellular membranes. The S protein is a target for neutralizing antibodies. Each vial contains approximately 100 ?L of NR-53589 in 10 mM HEPES, pH 7, 150 mM NaCl and 2 mM ethylenediamine-tetraacetic acid (EDTA).

NR-52724?? Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine and Twin-Strep? Tags, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine and Twin-Strep? Tags, Recombinant from HEK293 Cells|-60°C or colder|DM MagnaniAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine and Twin-Strep^? Tags, Recombinant from HEK293 Cells, NR-52724.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment. A recombinant form of the spike (S) glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QHD43416) was produced by transient transfection of the plasmid VRC7471 into human embryonic kidney HEK293 cells, purified by immobilized metal affinity and StrepTactin? Sepharose^? chromatography, and dialyzed into buffer. NR-52724 lacks the signal sequence and contains 1194 residues (ectodomain) of the SARS-CoV-2 S glycoprotein; the recombinant protein was stabilized by substitution at the furin S1/S2 cleavage site (RRAR→GSAS; residues 682 to 685) and KV→PP mutations (residues 986 and 987), and includes a T4 foldon trimerization domain, HRV3C protease cleavage site, and C-terminal octa-histidine and Twin-Strep^? (TST) tags. NR-52724 has a theoretical molecular weight of 140,700 daltons. The crystal structure for trimeric S glycoprotein from SARS-CoV-2 has been solved at 3.46 ? resolution (PDB: 6VSB). The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor allows fusion of the viral and cellular membranes. The S protein is a target for neutralizing antibodies. Each vial contains approximately 100 ?L of NR-52724 in phosphate buffered saline (PBS; pH 7.0). Note: The long-term stability of this preparation is not known at this time. It is recommended that users confirm the activity of the product if not used within three months of receipt. Additional information and tools are available at ViPR (Virus Pathogen Resource).