NR-53589来自SARS相关冠状病毒2的刺突糖蛋白(稳定）,Wuhan-Hu-1带有C末端组氨酸和Twin-Strep?标签,来自HEK293细胞(蛋白质）的重组
产品图片
货号/SKU
NR-53589
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质，其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类：美国、1
产品描述信息
NR-53589?? Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine and Twin-Strep? Tags, Recombinant from HEK293 Cells(Proteins)|SARS-Related Coronavirus 2|Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine and Twin-Strep? Tags, Recombinant from HEK293 Cells| -20°C or colder|DN Sather, P Myler, J McLellanAcknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Spike Glycoprotein (Stabilized) from SARS-Related Coronavirus 2, Wuhan-Hu-1 with C-Terminal Histidine and Twin-Strep? Tags, Recombinant from HEK293 Cells, NR-53589.”|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
A recombinant form of the spike (S) glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QHD43416) was produced by transient transfection of a previously published construct (VRC7471) into human embryonic kidney HEK293 cells and purified by immobilized metal affinity and gel filtration chromatography. NR-53589 lacks the native signal sequence and contains 1194 residues (ectodomain) of the SARS-CoV-2 S glycoprotein; the recombinant protein was stabilized by substitution at the furin S1/S2 cleavage site (RRAR→GSAS; residues 682 to 685) and KV→PP mutations (residues 986 and 987), and includes a T4 foldon trimerization domain, HRV3C protease cleavage site, and C-terminal octa-histidine and Twin-Strep? tags. NR-53589 has a theoretical molecular weight of 140,700 daltons. The crystal structure for trimeric S glycoprotein from SARS-CoV-2 has been solved at 3.46 ? resolution (PDB: 6VSB).
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor, allows fusion of the viral and cellular membranes. The S protein is a target for neutralizing antibodies.
Each vial contains approximately 100 ?L of NR-53589 in 10 mM HEPES, pH 7, 150 mM NaCl and 2 mM ethylenediamine-tetraacetic acid (EDTA).
A recombinant form of the spike (S) glycoprotein from severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), Wuhan-Hu-1 (GenPept: QHD43416) was produced by transient transfection of a previously published construct (VRC7471) into human embryonic kidney HEK293 cells and purified by immobilized metal affinity and gel filtration chromatography. NR-53589 lacks the native signal sequence and contains 1194 residues (ectodomain) of the SARS-CoV-2 S glycoprotein; the recombinant protein was stabilized by substitution at the furin S1/S2 cleavage site (RRAR→GSAS; residues 682 to 685) and KV→PP mutations (residues 986 and 987), and includes a T4 foldon trimerization domain, HRV3C protease cleavage site, and C-terminal octa-histidine and Twin-Strep? tags. NR-53589 has a theoretical molecular weight of 140,700 daltons. The crystal structure for trimeric S glycoprotein from SARS-CoV-2 has been solved at 3.46 ? resolution (PDB: 6VSB).
The S glycoprotein mediates viral binding to the host angiotensin converting enzyme 2 (ACE2). This protein forms a trimer, and when bound to a host receptor, allows fusion of the viral and cellular membranes. The S protein is a target for neutralizing antibodies.
Each vial contains approximately 100 ?L of NR-53589 in 10 mM HEPES, pH 7, 150 mM NaCl and 2 mM ethylenediamine-tetraacetic acid (EDTA).
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.此限制的订单将在发货前发送到NIAID批准.
来自严重急性呼吸综合征相关的冠状病毒2（SARS-COV-2），武汉-U-1（Genpept：）通过先前公布的构建体（VRC7471）进入人胚胎肾HEK293细胞并通过固定化的金属亲和力和凝胶过滤色谱法纯化. NR-53589缺乏天然信号序列，含有1194个残基（Ectodomain）的SARS-COV-2 S糖蛋白;通过在Furin S1 / S2切割位点（RrAR→GSAs;残基682至685）和KV→PP突变（残基986和987）中的替代物稳定重组蛋白，并且包括T4粘性三聚化结构域，HRV3C蛋白酶切割位点，和C末端Octa-mostatinine和Twin-strep ?标签. NR-53589具有140,700道尔顿的理论分子量. SARS-COV-2中三聚糖蛋白的晶体结构已在3.46埃分辨率（PDB： 6vsb ）.
S糖蛋白介导与宿主血管紧张素转化酶2（ACE2）的病毒结合.该蛋白质形成三聚体，并且当与宿主受体结合时，允许融合病毒和细胞膜. S蛋白是中和抗体的靶标.
每个小瓶在10mM Hepes，pH 7,150mM NaCl和2mM乙二胺 - 四乙酸（EDTA）中含有约100μl的NR-53589.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立，旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中，可以监控科学界对这些材料的访问和使用，并确保试剂的质量控制。
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BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月， ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料，包括由其他政府支持的研究项目存放的材料，将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中，涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
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