NR-32875鼠疫耶尔森菌,LcrV蛋白,大肠杆菌重组体(蛋白质)
产品图片
货号/SKU
NR-32875
货号/规格
EA
库存与交货期
3-8周
人民币价格
14000
试剂海关审批
A/B级风险物质只能直接使用人购买并持有实验室有效资质,其它询客服确认
国外采购
支持/部分限制一年内购买数量
厂牌
BEI Resources(ATCC)
产品基础信息
生物安全等级建议分类:美国、1
产品描述信息
NR-32875??Yersinia pestis, LcrV Protein, Recombinant from Escherichia coli(Proteins)|Yersinia pestis|LcrV Protein, Recombinant from Escherichia coli|-20°C or colder|BEI ResourcesAcknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Yersinia pestis LcrV Protein, Recombinant from Escherichia coli, NR-32875."|Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.
Yersinia pestis (Y. pestis), the causative agent of the plague, secretes massive amounts of LcrV (low-calcium-response V or V antigen) during infection. Mutations that abrogate the expression of LcrV render Y. pestis avirulent. LcrV is a multifunctional protein that is central to the activity of the type III secretion apparatus of Y. pestis. It has no known catalytic function, and its biological activity is dependent on interactions with other proteins. Injection of LcrV into animals stimulates humoral responses that offer protection against plague infection. The amino acid sequence for LcrV from Y. pestis has been reported by 2 groups in the NCBI protein database (AAC62574 and AAC69799). The crystal structure for LcrV from Y. pestis has been solved at 2.17 ? resolution (PDB: 1R6F).
Recombinant LcrV protein (Y. pestis, strain KIM5) was expressed in Escherichia coli BL21 (DE3) cells as described. The N-terminal deca-histidine tagged protein was purified via nickel-NTA and gel filtration chromatography. Cleavage with Factor Xa resulted in a protein with one extra histidine at the amino acid terminus. The protein preparation was treated with Triton X-114 to reduce endotoxin contamination. NR-32875 has the same amino acid sequence as NR-3832 and was expressed and purified using similar methodology but was produced at BEI Resources, not the University of Chicago.
Each vial contains approximately 1 mg of recombinant LcrV protein in PBS, pH 7.4.
Recombinant LcrV protein (Y. pestis, strain KIM5) was expressed in Escherichia coli BL21 (DE3) cells as described. The N-terminal deca-histidine tagged protein was purified via nickel-NTA and gel filtration chromatography. Cleavage with Factor Xa resulted in a protein with one extra histidine at the amino acid terminus. The protein preparation was treated with Triton X-114 to reduce endotoxin contamination. NR-32875 has the same amino acid sequence as NR-3832 and was expressed and purified using similar methodology but was produced at BEI Resources, not the University of Chicago.
Each vial contains approximately 1 mg of recombinant LcrV protein in PBS, pH 7.4.
主要内容
此项目的每个订单数量限制为1.此商品每年可订购两次.在此限制上订单将在发货前发送到NIAID进行批准. yersinia pestis(y.pestis),致病代理人瘟疫,在感染期间分泌大量的LCRV(低钙响应V或V抗原).消除了LCRV表达的突变呈现 y. Pestis 无毒. LCRV是一种多功能蛋白质,其是III型分泌装置的活性的核心. Pestis.它没有已知的催化功能,其生物活性依赖于与其他蛋白质的相互作用.将LCRV注射到动物刺激了对瘟疫感染的保护的体液反应.来自 Y的LCRV的氨基酸序列. Pestis 已在NCBI蛋白质数据库(AAC62574和AAC69799)中报道了2组.来自 y的LCRV的晶体结构. Pestis 已经解决了2.17埃分辨率(PDB:1R6F).重组LCRV蛋白( Y. Pestis,菌株Kim5)在大肠杆菌中表达如上所述 BL21(DE3)电池.通过镍-NTA和凝胶过滤色谱法纯化N-末端Deca-组氨酸标记的蛋白.用因子XA的裂解导致氨基酸末端的蛋白质有一个额外的组氨酸.用Triton X-114处理蛋白质制剂以减少内毒素污染. NR-32875具有与NR-3832相同的氨基酸序列,并使用类似的方法表达和纯化,但在Bei Resources,而不是芝加哥大学. 每个小瓶含有约1mg重组LCRV蛋白在PBS,pH 7.4中.
厂牌介绍
BEI Resources 由美国国家过敏和传染病研究所 ( NIAID )成立,旨在为研究 A、B 和 C 类优先病原体、新兴传染病病原体、非病原微生物和其他相关微生物材料提供试剂、工具和信息到研究界。BEI Resources 获取、验证和生产科学家进行基础研究和开发改进的诊断测试、疫苗和疗法所需的试剂。通过将这些功能集中在 BEI Resources 中,可以监控科学界对这些材料的访问和使用,并确保试剂的质量控制。
除了为传染病界提供材料外,BEI Resources 还鼓励和支持研究人员和机构的材料存放。使用 BEI Resources存放材料对研究人员和研究社区有许多优势,包括安全存储、社区访问和分发;同时保护存款人的知识产权。只要有需要,BEI 资源库将作为研究人员的资源进行维护。您在 BEI Resources 的存款是一项有助于未来研究的长期投资。
BEI Resources 自 2003 年起由美国典型培养物保藏中心 (ATCC) 根据合同管理。2016 年 5 月, ATCC获得了一份为期七年的继续管理 BEI Resources的合同。合同范围已扩大到更全面的研究目录材料,包括由其他政府支持的研究项目存放的材料,将提供给生物防御和新兴传染病科学界。真菌、寄生虫、载体和其他相关材料已添加到现有的细菌、病毒和毒素试剂中,涵盖 NIAID A、B 和 C 类优先病原体和 NIAID 指定的新发传染病病原体和生物。
品牌标识
可能感兴趣的内容
2022-04-01
2021-12-21
2022-03-31
2022-04-01
2022-04-01
2021-12-21
2021-12-21
2021-10-31
2022-04-01
2024-05-19
2022-04-01
2024-01-21
2024-05-19
2022-01-10