LcrV 蛋白

Yersinia pestis (Y. pestis), the causative agent of the plague, secretes massive amounts of LcrV (low-calcium-response V or V antigen) during infection. Mutations that abrogate the expression of LcrV render Y. pestis avirulent. LcrV is a multifunctional protein that is central to the activity of the type III secretion apparatus of Y. pestis. It has no known catalytic function, and its biological activity is dependent on interactions with other proteins. Injection of LcrV into animals stimulates humoral responses that offer protection against plague infection. The amino acid sequence for LcrV from Y. pestis has been reported by 2 groups in the NCBI protein database (AAC62574 and AAC69799). The crystal structure for LcrV from Y. pestis has been solved at 2.17 ? resolution (PDB: 1R6F).

Recombinant LcrV protein (Y. pestis, strain KIM5) was expressed in Escherichia coli BL21 (DE3) cells as described. The N-terminal deca-histidine tagged protein was purified via nickel-NTA and gel filtration chromatography. Cleavage with Factor Xa resulted in a protein with one extra histidine at the amino acid terminus. The protein preparation was treated with Triton X-114 to reduce endotoxin contamination. NR-32875 has the same amino acid sequence as NR-3832 and was expressed and purified using similar methodology but was produced at BEI Resources, not the University of Chicago.

Each vial contains approximately 1 mg of recombinant LcrV protein in PBS, pH 7.4.